N N (Publications)
Publisher Walter De Gruyter • Berlin • Boston.
Publication Type Journal
Publication Title Acetylcholinesterase (AChE) Inhibitory Activity, Antioxidant Properties And Phenolic Composition Of Two Aframomum Species
Publication Authors Stephen A. Adefegha and Ganiyu Oboh
Year Published 2012
Abstract

 Abstract
  Background :   Aframomum   species  are  widely  used  as  a
food  supplement  and  remedy  in  folklore  medicine  for  the
management  of  several  diseases.  This  study  was  designed
to  investigate  the  acetylcholinesterase  inhibitory  activity
and  antioxidant  properties  of  phenolic-rich  extracts  from
two  Aframomum  species:  Aframomum danielli  (Hook F.) K.
Schum (Zingiberaceae) and  Aframomum   melegueta  (Roscoe)
K. Schum (Zingiberaceae) seeds.
  Methods : Acetylcholinesterase inhibitory activity and anti-
oxidant properties [inhibition of quinolinic acid (QA)-induced
lipid peroxidation in rat brain, reducing properties, 2,2-azinobis
(3-ethylbenzo-thiazoline-6-sulfonate) (ABTS) and 1,1-diphe-
nyl-2-picrylhydrazyl  (DPPH)  radical  scavenging  abilities]
using  in  vitro  models  were  evaluated.  Phenolic  composi-
tion  of  the  seed  extracts  using  reversed  phase  high  perfor-
mance chromatography (RP-HPLC) and gas chromatography
coupled with fl ame ionization detector (GC-FID) were also
assessed.
  Results : Both extracts exhibited acetylcholinesterase inhibi-
tory activity in a dose-dependent manner (125 – 1000   μ  g/mL);
however,  A. melegueta  extract (IC
50
    =   373.33   μ  g/mL) had a
signifi cantly  higher  (p   <   0.05)  acetylcholinesterase  inhibi-
tory activity than  A. danielli  extract (IC
50
    =   417.10   μ  g/mL).
Furthermore,  both  extracts  signifi cantly  decreased  QA-
elevated  brain  malondialdehyde  (MDA)  contents,  reduced
Fe  3 +     to  Fe  2 +     and  scavenged  DPPH  and  ABTS  radicals.
Phenolic  characterization  of  the  seeds  by  RP-HPLC  at
280 nm showed abundance of quercetin and kaempferol in
 A.  melegueta   and  chlorogenic  acid  in   A.  danielli ,  whereas
GC-FID revealed that p-hydroxybenzoic acid was abundant
in both seeds.
  Conclusions : Inhibitory effect of these extracts on acetylcho-
linesterase  activity  and  their  antioxidant  property  could  be

attributed to the combined effect of phenolic and non-pheno-
lic constituents of the seeds. These effects could be part of the
possible biochemical mechanism by which these seeds elicit
their  protection  against  oxidative  stress  in  brain;  however,
 A. melegueta  showed the more promising potential. 


Publisher Oboh G., Adefegha S.A., Ademosun A.O., And Unu D.
Publication Type Others
Publication Title Effects Of Hot Water Treatment On The Phenolic Phytochemicals And Antioxidant Activities Of Lemon Grass (Cymbopogon Citratus)
Publication Authors Oboh G., Adefegha S.A., Ademosun A.O., and Unu D.
Year Published 2010
Abstract nil
Publisher Oboh G., Ademosun A. O., Adefegha S. A. And Lajide L.
Publication Type Others
Publication Title Enhancement Of Antioxidant Properties Of Citrus Peels Using Aspergilus Niger Solid Substrate Fermentation Technology.
Publication Authors Oboh G., Ademosun A. O., Adefegha S. A. and Lajide L.
Year Published 2010
Abstract

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Publisher Oboh, G., Adefegha, S. A., Akomolafe, T. L., And Adetuyi A. O.
Publication Type Others
Publication Title Protective Effects Of African Locust Beans (Parkia Filicoidea) Fruit Husk Extract Against Cyclophosphamide-induced Oxidative Stress In Rats Brain.
Publication Authors Oboh, G., Adefegha, S. A., Akomolafe, T. L., and Adetuyi A. O.
Year Published 2010
Abstract

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Publisher Oboh, G And Adefegha, S. A.
Publication Type Others
Publication Title Nutritional Properties Of Plantain- Based Biscuits.
Publication Authors Oboh, G and Adefegha, S. A.
Year Published 2010
Abstract

NIL


Publisher Oboh, G., Akomolafe, T. L., Adefegha, S. A. And Adetuyi A. O.
Publication Type Others
Publication Title Attenuation Of Cyclophosphamide-induced Neurotoxicity In Rat By Yellow Dye Extract From Root Of Brimstone Tree (Morinda Lucida).
Publication Authors Oboh, G., Akomolafe, T. L., Adefegha, S. A. and Adetuyi A. O.
Year Published 2010
Abstract

NIL


Publisher Oboh, G., Adefegha, S. A., Akomolafe, T. L., And Adetuyi A. O.
Publication Type Others
Publication Title Dietary Supplementation Of Pterocarpus Erinaceus (African Rosewood) Dye Extract Ameliorates Cyclophosphamide - Induced Toxicity In Rat’s Brain And Liver.
Publication Authors Oboh, G., Adefegha, S. A., Akomolafe, T. L., and Adetuyi A. O.
Year Published 2010
Abstract

NIL


Publisher Oboh, G., Akomolafe, T. L., Adefegha, S. A. And Adetuyi A. O.
Publication Type Others
Publication Title Ameliorative Effect Of Ethanolic Extract Of Nigerian Zanthoxylum (Zanthoxylum Zanthoxyloides) Stem Against Cyclophosphamide Induced Oxidative Stress In Rat’s Brain.
Publication Authors Oboh, G., Akomolafe, T. L., Adefegha, S. A. and Adetuyi A. O.
Year Published 2009
Abstract

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