Akindahunsi Afolabi Akintunde (Publications)
Publisher Akindahunsi, A.A., Adewusi, S.R.A., Torimiro, S.E.A. And Oke, O.L.
Publication Type Journal
Publication Title ). Food Composition And Endemic Goitre In Akungba And Oke-Agbe Of Southwestern Nigeria
Publication Authors Akindahunsi, A.A., Adewusi, S.R.A., Torimiro, S.E.A. and Oke, O.L.
Year Published 1993
Abstract

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Publisher Akindahunsi, A.A; Purgicky, A; Goldhar, J; Ofek, I. And Sharon, N.
Publication Type Journal
Publication Title ). Purification Of The Inhibitory Glycoproteins In Peritoneal Effluent From Patients Treated With Peritoneal Dialysis
Publication Authors Akindahunsi, A.A; Purgicky, A; Goldhar, J; Ofek, I. And Sharon, N.
Year Published 1996
Abstract

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Publisher Akindahunsi, A. A; Grissom, F.E., Adewusi, S.R.A; Afolabi, O.A; Torimiro, S.E.A. And Oke. O.L
Publication Type Journal
Publication Title Parameters Of Thyroid Function In The Endemic Goitre Of Akungba And Oke–Agbe Villages Of Akoko Area Of Southwestern Nigeria
Publication Authors Akindahunsi, A. A; Grissom, F.E., Adewusi, S.R.A; Afolabi, O.A; Torimiro, S.E.A. and Oke. O.L
Year Published 1998
Abstract

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Publisher Akindahunsi, A.A. And Oboh, G
Publication Type Journal
Publication Title Chemical Studies On Two Tropical Wild Yams Dioscorea Bulbifera And Dioscorea Manganotiana
Publication Authors Akindahunsi, A.A. and Oboh, G
Year Published 1998
Abstract

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Publisher Akindahunsi, A.A., Oboh, G. And Oshodi, A.A
Publication Type Journal
Publication Title ). Effect Of Fermenting Cassava With Rhizopus Oryzae On The Chemical Composition Of Its Flour And Gari Products.
Publication Authors Akindahunsi, A.A., Oboh, G. and Oshodi, A.A
Year Published 1999
Abstract

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Publisher Akindahunsi, A. A. And Adewusi, S.R.A.
Publication Type Journal
Publication Title Cassava, Protein And Iodine Interaction In The Thyroid Status Of Rats
Publication Authors Akindahunsi, A. A. and Adewusi, S.R.A.
Year Published 2000
Abstract

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Publisher Oboh, G; Akindahunsi, A. A. And Oshodi, A. A.
Publication Type Journal
Publication Title Aflatoxin And Moisture Contents Of Micro-fungi Fermented Cassava Products (flour And Gari).
Publication Authors Oboh, G; Akindahunsi, A. A. and Oshodi, A. A.
Year Published 2000
Abstract

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Publisher Oboh, G; Akindahunsi, A.A And Oshodi, A.A.
Publication Type Journal
Publication Title ) Nutrient And Anti-nutrient Contents Of Aspergillus Niger-fermented Cassava Products (flour And Gari).
Publication Authors Oboh, G; Akindahunsi, A.A and Oshodi, A.A.
Year Published 2002
Abstract

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Publisher Bandiera, A; Tell, G; Marsich, E; Scaloni, A. Pocsfalvi, G; Akindahunsi, A.A; Cesaratto, L. And Manzini, G.
Publication Type Journal
Publication Title Cytosine-block Telomeric Type DNA-binding Activity Of HnRNP Proteins From Human Cell Lines
Publication Authors Bandiera, A; Tell, G; Marsich, E; Scaloni, A. Pocsfalvi, G; Akindahunsi, A.A; Cesaratto, L. and Manzini, G.
Year Published 2003
Abstract

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Publisher Akindahunsi, A. A. And Oboh, G
Publication Type Journal
Publication Title Effect Of Fungi Fermentation On The Organoleptic Property, Energy Content And In Vitro Multienzyme Digestibility Of Cassava Products (flour And Gari)
Publication Authors Akindahunsi, A. A. and Oboh, G
Year Published 2003
Abstract

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Publisher Olaleye, M. T; Akindahunsi, A. A And Ogunlade A. O
Publication Type Journal
Publication Title ) Effects Of Aqueous- Alcoholic Extract Of Persea Americana (leaves) On Isolated Rabbit Heart Preparation And Blood Pressure Of Normotensive Rats
Publication Authors Olaleye, M. T; Akindahunsi, A. A and Ogunlade A. O
Year Published 2003
Abstract

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Publisher Akindahunsi, A.A., Oboh G. And Adetuyi, F.C.
Publication Type Journal
Publication Title ) Influence Of Change In Relative Humidity On The Storage Stability And Microbial Quality Of Micro-fungi Fermented Cassava Product (gari).
Publication Authors Akindahunsi, A.A., Oboh G. and Adetuyi, F.C.
Year Published 2003
Abstract

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Publisher Oboh G. And Akindahunsi , A.A.
Publication Type Journal
Publication Title Chemical Changes In Cassava Peels Fermented With Mixed Culture Of Aspergillus Niger & Two Species Of Lactobacillus Integrated Bio-system
Publication Authors Oboh G. and Akindahunsi , A.A.
Year Published 2003
Abstract

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Publisher Olaleye, M.T And Akindahunsi, A.A
Publication Type Journal
Publication Title Phytochemical Investigation And Antimicrobial Activities Of Extracts Of The Leaves Of Viscum Album
Publication Authors Olaleye, M.T and Akindahunsi, A.A
Year Published 2003
Abstract

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Publisher Salawu, S.O., Adu, O.C. And Akindahunsi, A.A
Publication Type Journal
Publication Title Effects Of Processing On The Nutrients Composition Of A Selected Fresh And Brackish Water African Catfish (Clarias Gariepinus) In Ondo State Nigeria.
Publication Authors Salawu, S.O., Adu, O.C. and Akindahunsi, A.A
Year Published 2004
Abstract

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Publisher Oboh, G And Akindahunsi, A.A
Publication Type Journal
Publication Title Changes In The Ascorbic Acid, Total Phenol And Antioxidant Activity Of Sun-Dried Green Leafy Vegetables In Nigeria.
Publication Authors Oboh, G and Akindahunsi, A.A
Year Published 2004
Abstract

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Publisher Antonella Bandiera, Nevenka Medic, A. Akintunde Akindahunsi And Giorgio Manzini
Publication Type Journal
Publication Title In Vitro Dual Binding Activity Of A Evolutionarily Related Subgroup Of HnRNP Proteins
Publication Authors Antonella Bandiera, Nevenka Medic, A. Akintunde Akindahunsi and Giorgio Manzini
Year Published 2005
Abstract

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Publisher Oboh, G And Akindahunsi, A.A
Publication Type Journal
Publication Title ) Nutritional And Toxicological Evaluation Of Zaccharomyces Cerevisae Fermented Cassava Flour.
Publication Authors Oboh, G and Akindahunsi, A.A
Year Published 2005
Abstract

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Publisher Akindahunsi, A. A.and Salawu S. O
Publication Type Journal
Publication Title Phytochemical Screening And Nutrient-Antinutrient Composition Of Selected Tropical Green Leafy Vegetables
Publication Authors Akindahunsi, A. A.and Salawu S. O
Year Published 2005
Abstract

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Publisher Salawu, S.O., Akindahunsi, A.A And Comuzzo, P
Publication Type Journal
Publication Title Chemical Composition And In Vitro Antioxidant Activities Of Some Nigerian Vegetables.
Publication Authors Salawu, S.O., Akindahunsi, A.A and Comuzzo, P
Year Published 2006
Abstract

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Publisher University Of Nigeria, Nsukka, Nigeria
Publication Type Conference
Publication Title 21st Annual Conference Of The Nigerian Society Of Biochemistry And Molecular Biology
Publication Authors University of Nigeria, Nsukka, Nigeria
Year Published 2001
Abstract

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Publisher Ahmadu Bello University, Zaria, Nigeria
Publication Type Conference
Publication Title 22nd Annual Conference Of The Nigerian Society Of Biochemistry And Molecular Biology
Publication Authors Ahmadu Bello University, Zaria, Nigeria
Year Published 2002
Abstract

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Publisher University Of Ado-Ekiti, Nigeria
Publication Type Conference
Publication Title 17th Annual Conference Of Biotechnology Society Of Nigeria (BSN)
Publication Authors University of Ado-Ekiti, Nigeria
Year Published 2004
Abstract

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Publisher Adewusi, S.R. A; Akindahunsi, A.A. And Oke, O.L.
Publication Type Conferenceproceeding
Publication Title Cassava Processing, Consumption And Human Health
Publication Authors Adewusi, S.R. A; Akindahunsi, A.A. and Oke, O.L.
Year Published 1993
Abstract

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Publisher Akindahunsi, A.A. And Oboh, G.
Publication Type Conferenceproceeding
Publication Title Chemical Studies On Two Tropical Wild Yams, Dioscorea Bulbifera And Dioscorea Manganotiana Proceedings Of The 22nd Annual NIFST Conference
Publication Authors Akindahunsi, A.A. and Oboh, G.
Year Published 1998
Abstract

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Publisher Akindahunsi, A.A. And Oboh, G.
Publication Type Conferenceproceeding
Publication Title Akindahunsi, A.A. And Oboh, G.
Publication Authors Akindahunsi, A.A. and Oboh, G.
Year Published 2000
Abstract

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Publisher Oboh G. And Akindahunsi, A.A.
Publication Type Conferenceproceeding
Publication Title Safety Evaluation Of Aspergillus Flavus Fermented Cassava Flour (Manihot Esculenta, Crantz). In Proceedings Of 16th Annual Conference Of Biotechnology Society Of Nigeria
Publication Authors Oboh G. and Akindahunsi, A.A.
Year Published 2003
Abstract

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Publisher Oseni, O. A., Ekperigin, M. M., Akindahunsi, A. A. And Oboh, G
Publication Type Journal
Publication Title Studies Of Physiochemical And Microbial Properties Of Soilsfrom Rainforest And Plantation In Ondo State, Nigeria.
Publication Authors Oseni, O. A., Ekperigin, M. M., Akindahunsi, A. A. and Oboh, G
Year Published 2007
Abstract

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Publisher Salawu SO, Akindahunsi AA
Publication Type Journal
Publication Title Protective Effect Of Some Tropical Vegetables Against CCl4-induced Hepatic Damage.
Publication Authors Salawu SO, Akindahunsi AA
Year Published 2007
Abstract

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Publisher Oyetayo, F.L., Akindahunsi A.A And Oyetayo V.O
Publication Type Journal
Publication Title Chemical Profile And Amino Acids Composition Of Edible Mushrooms Pleurotus Sajor-Caju.
Publication Authors Oyetayo, F.L., Akindahunsi A.A and Oyetayo V.O
Year Published 2007
Abstract

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Publisher Oyetayo, F.L., Akindahunsi A.A And Oyetayo V.O
Publication Type Journal
Publication Title Chemical Profile And Amino Acids Composition Of Edible Mushrooms Pleurotus Sajor-Caju.
Publication Authors Oyetayo, F.L., Akindahunsi A.A and Oyetayo V.O
Year Published 2007
Abstract

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Publisher Oboh G, Ademiluyi A. O And Akindahunsi A. A.
Publication Type Journal
Publication Title Changes In The Polyphenols And Antioxidant Activity During Fermentation Of Some Underutilized Legumes.
Publication Authors Oboh G, Ademiluyi A. O and Akindahunsi A. A.
Year Published 2008
Abstract

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Publisher Akindahunsi A. A.
Publication Type Journal
Publication Title R-α–Lipoic Acid And Acetyl-L-carnitine Optimal Combinations In MPP+- Induced Cellular Model Of Parkinson’s Disease,
Publication Authors Akindahunsi A. A.
Year Published 2009
Abstract

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Publisher Studium Press LLC. USA
Publication Type Books
Publication Title Some Serum Biochemical Indices Of Wistar Albino Rats Given Partially Purified Methanolic Extract Of The Leaves Of Persea Americana. In: Plant Bioactives In Traditional Medicine, Recent Progress In Medicinal Plants Series (D. K. Majumdar, J. N. Govil, V. K. Singh & Rajeev Kumar Sharma, Eds.
Publication Authors Olaleye,M.T and Akindahunsi, A.A
Year Published 2004
Abstract

Nil


Publisher Daya Publishing House Delhi-110035
Publication Type Books
Publication Title Antioxidant And Antihypertensive Investigation Of Seed Extract Of Parinari Curatellifolia. In: Medicinal Plants: Phytochemistry, Pharmacology And Therapeutics (V.K Gupta & C,D Singh Surjeet Singh, A.Kaul
Publication Authors Olaleye, M.T, Adegboye, O.O and Akindahunsi, A.A
Year Published 2010
Abstract

Nil


Publisher Graceland Prints, Nigeria
Publication Type Books
Publication Title Biotechnology In Functional Food Products Development And Delivery. In: Oboh G (Ed). Functional Foods: Unlocking The Medicine In Foods
Publication Authors Akindahunsi A. A
Year Published 2017
Abstract

Nil


Publisher Taylor And Francis
Publication Type Journal
Publication Title Cassava Processing, Consumption And Cyanide Toxicity
Publication Authors Adewusi, S. R.A. and Akindahunsi, A.A
Year Published 1994
Abstract

The frequency of cassava consumption was investigated among three groups of people representing students from traditional and nontraditional cassava-consuming environments and cassava processors. Of these, 64% of the students at Obafemi Awolowo University, Ile-Ife, 38% of those at the Federal University of Technology, Akure, and 44% of the cassava processors consumed cassava products at least once a day, while 4, 35, and 28% of the groups, respectively, were moderate consumers (4-6 times a week). The serum thiocyanate level of the processors was significantly higher (0.57 mg/dl) than those of the students (0.38 mg/dl), but there was no significant difference in the urinary thiocyanate level of the three groups. Analysis of cassava and its intermediate and final products for free cyanide, acetone cyanohydrin, and intact glucosides during the production of such cassava products as gari, fufu, and lafun revealed that while the finished products might be safe for human consumption, the workers were probably exposed at different stages of processing to nondietary sources of cyanide


Publisher Springer: Dordrecht, The Netherlands
Publication Type Books
Publication Title On The Question Of Convergent Evolution In Biochemistry. In: Seckbach, J., Chela-Flores, J., Owen, T. And Raulin, F (Eds). “Life In The Universe”, Cellular Origin And Life In Extreme Habitats And Astrobiology
Publication Authors Akindahunsi, A. A. and Chela-Flores, J
Year Published 2004
Abstract
Convergent evolution is an ubiquitous phenomenon, since it occurs at the levels of morphology, physiology, behavior (Eisthen and Nishikawa, 2002), and at the molecular level (Pace, 2001). Evolutionary convergence is significant for the central problem of astrobiology. Since all forms of life known to us are terrestrial, it is relevant to question whether the science of biology is of universal validity (Dawkins, 1983), and whether the molecular events that were precursors of the origin of life are bound to occur elsewhere in the universe wherever conditions are similar to the terrestrial ones. We discuss evolutionary convergence and its classification into functional, mechanistic, structural and sequence convergence (Doolittle, 1994), which should help us in the context of astrobiology defining bioindicators in the exploration of the solar system

Publisher Nil
Publication Type Journal
Publication Title In Vitro Studies On The Steroidal Extracts Of Two Wild Yams (Dioscorea Spp)
Publication Authors Oboh, G; Akindahunsi, A.A. and Ekperigin, M. M
Year Published 1997
Abstract

Dioscorea bulbifera and Dioscorea manganotiana were evaluated for their potential as a source of saponin and sapogenin. The levels of these steroid hormone precursors were determined by solvent extraction and characterized by froth test, haemolytic test, colour, taste and TLC analysis. The saponin contents of both yams were 1.04±0.08% {Dioscorea bulbifera) and 1.58±0.26% {Dioscorea manganotiana). The sapogenin content oi Dioscorea manganotiana was 6.04±0.06mg/g, while that of dioscorea bulbifera was 3.36±0.37mg/g. The saponin had a dark-brown colour, bitter taste, frothing ability and haemolysed blood. TLC analysis gave a purple spot with Rf ranging from 0.55 to 0.56. Since the wild yams used for the present study are neither consumed by man nor used for livestock feeding, coupled with their relative abundance and low cost, they hold a good promise with respect to sourcing precursors for commercial production of steroid hormones.


Publisher Nil
Publication Type Journal
Publication Title Antimicrobial Studies On Saponin Extracts From Two Wild Yams (Dioscorea Spp)
Publication Authors Oboh, G; Akindahunsi, A.A; Famutimi, R. and Adetuyi, F. C.
Year Published 1998
Abstract

Nil


Publisher La Rivista Italiana Delle Sostanze Grasse, Italy
Publication Type Journal
Publication Title Effect Of Some Post-harvest Treatments On The Bioavailability Of Zinc From Some Tropical Vegetables.
Publication Authors Akindahunsi, A. A. and Oboh, G.
Year Published 1999
Abstract

The effect of three post-harvest treatments namely, sun-drying, oven-drying (75 and 100°C) and blanching at 100°C for 5 min on the bioavailability of zinc from some selected tropical vegetables was investigated. Phytate, zinc and calcium contents of six vegetables namely, cock's comb, water leaf, long fruited jute, bitter leaf, Indian spinach and bush green were analyzed and their phytate: Zn, Ca:phytate and [Ca][phytate]/[Zn] molar ratios calculated. Though the phytate levels (mg/100 g) obtained were high (1128.2-2752.9), the levels were further increased by the post-harvest treatments except blanching which actually brought about reduction (33.3-65.0%). On the other hand, the calculated phytate : Zn molar ratio was generally lower than the critical value of 15:1. However, while sun-drying and oven-drying at both 75 and 100°C gave high figures for the calculated [Ca][phytate]/[Zn] molar ratios, 5 min blanching at 100°C gave normal figures for this ratio, except water leaf. Bitter leaf (Vernonia amygdalina Del) recorded the least values for the three ratios, thus indicating this vegetable as the best source of zinc. The results of the present study indicate a reduced zinc bioavailability from vegetables by such post-harvest treatments as sun-drying and oven-drying compared to the excellent performance of blanching.


Publisher Nil
Publication Type Journal
Publication Title Biotransformation Of Steroidal Saponins From Two Wild Yams (Dioscorea Spp) To Sapogenins
Publication Authors Oboh, G; Akindahunsi, A.A. and Ekperigin, M.M
Year Published 2000
Abstract

Dioscorea bulbifera and Dioscorea manganotiana were evaluated for their potential as a source of saponin and sapogenin. The levels of these steroid hormone precursors were determined by solvent extraction and characterized by froth test, haemolytic test, colour, taste and TLC analysis. The saponin contents of both yams were 1.04±0.08% {Dioscorea bulbifera) and 1.58±0.26% {Dioscorea manganotiana). The sapogenin content of Dioscorea manganotiana was 6.04±0.06mg/g, while that of dioscorea bulbifera was 3.36±0.37mg/g. The saponin had a dark-brown colour, bitter taste, frothing ability and haemolysed blood. TLC analysis gave a purple spot with Rf ranging from 0.55 to 0.56. Since the wild yams used for the present study are neither consumed by man nor used for livestock feeding, coupled with their relative abundance and low cost, they hold a good promise with respect to sourcing precursors for commercial production of steroid hormones


Publisher Nahrung, Germany
Publication Type Journal
Publication Title Investigation Of The Bioavailability Of Zinc And Calcium From Some Tropical Tubers.
Publication Authors Oladimeji, M.O; Akindahunsi, A.A. and Okafor, A
Year Published 2000
Abstract

Nil


Publisher Springer: Dordrecht, The Netherlands
Publication Type Books
Publication Title On The Question Of Convergent Evolution In Biochemistry
Publication Authors Akindahunsi, A. A. and Chela-Flores, J
Year Published 2004
Abstract
Convergent evolution is an ubiquitous phenomenon, since it occurs at the levels of morphology, physiology, behavior (Eisthen and Nishikawa, 2002), and at the molecular level (Pace, 2001). Evolutionary convergence is significant for the central problem of astrobiology. Since all forms of life known to us are terrestrial, it is relevant to question whether the science of biology is of universal validity (Dawkins, 1983), and whether the molecular events that were precursors of the origin of life are bound to occur elsewhere in the universe wherever conditions are similar to the terrestrial ones. We discuss evolutionary convergence and its classification into functional, mechanistic, structural and sequence convergence (Doolittle, 1994), which should help us in the context of astrobiology defining bioindicators in the exploration of the solar system

Publisher A B Academic Publishers. Printed In Great Britain
Publication Type Journal
Publication Title Prediction Of Nutritional Status By Chemical Analysis Of Urine And Anthropometric Methods.
Publication Authors Adewusi, S.R.A; Torimiro, S.E.A. and Akindahunsi, A.A
Year Published 2002
Abstract

A combination of anthropometric and chemical indices was used to investigate the nutritional status of 26 healthy (H) and kwashiorkor (K) children aged 2-5 years and possibly predict the onset of malnutrition. The healthy children had significantly (p>0.05) higher values for weight, height, middle upper arm circumference and weight/height ratio than kwashiorkor children. The healthy children had significantly higher urinary concentrations of urea, inorganic sulphate and sulphate relative to creatinine, but significantly lower thiocyanate and thiocyanate relative to creatinine than the kwashiorkor children. The results of the present study would tend to indicate that a chemical analysis especially of urea, inorganic sulphate or thiocyanate alone or the urea/creatinine, inorganic sulphate/creatinine or thiocynate/creatinine ratio could be used to predict malnutrition after the initial anthropometric measurements. This study should however be repeated with a large population of volunteers to determine the specific cut-off points for each anthropometric and chemical analysis.


Publisher Nil
Publication Type Journal
Publication Title Effect Of Some Post-harvest Treatments On The Phytate And Tannin Contents Of Some Tropical Peppers (Capsicum Spp)
Publication Authors Akindahunsi, A. A. and Oboh, G
Year Published 2000
Abstract

Nil


Publisher Elsevier
Publication Type Journal
Publication Title Biochemical Changes In Cassava Products (flour & Gari) Subjected To Saccharomyces Cerevisae Solid Media Fermentation.
Publication Authors Oboh, G and Akindahunsi, A.A.
Year Published 2002
Abstract

In an attempt to enhance the nutritional quality of cassava products (flour and gari), Saccharomyces cerevisae was used in the fermentation (solid media) of cassava pulp. The mash obtained was subsequently processed into flour and gari, the forms in which cassava products are popularly consumed in Nigeria. These products were analysed with regard to proximate composition, mineral composition and antinutrient content. The result revealed that there were significant increases in the protein [flour (10.9%), gari (6.3%)] and fat [flour (4.5%), gari (3.0%)] contents. Conversely, there were significant decreases in the cyanide content [flour (9.5 mg/kg), gari (9.1 mg/kg)], carbohydrate [flour (77.9%), gari (84.5%)] and mineral (Zn, Mg, Fe, Ca, Na and K) contents of the cassava products, except in the fermented gari where there was significant increase in the Mg and Fe contents. However, Saccharomyces cerevisae fermentation of the cassava did not bring about any significant changes in the tannin, crude fibre or ash contents of the cassava products. Heavy metals, such as Cu, Ni and Pb, were not detected in either the fermented or unfermented cassava products. Furthermore, nutrient increase was higher in cassava flour while the antinutrient decrease was higher in gari. It could be inferred that Saccharomyces cerevisae, a cheap and non-pathogenic saprophytic aerobe, could be used for enhancing the nutritional potential of cassava products by increasing nutrient (protein and fat) and decreasing cyanide contents


Publisher Elsevier
Publication Type Journal
Publication Title Toxicological Investigation Of Aqueous Methanolic Extract Of The Calyces Of Hibiscus Sabdariffa L.
Publication Authors Akindahunsi, A.A and Olaleye, M.T.
Year Published 2003
Abstract

The aqueous fraction of an aqueous-alcoholic extract of Hibiscus sabdariffa L. calyces was given to Wistar albino rats (150-200g) orally as drugs to study the toxicity of the extract. The rats, which were fed with commercial diet and water ad libitum, were divided into six groups of four rats each. Treatments 1 through 6 received 0, 1, 3, 5, 10 and 15 doses of 250 mg/kg each, respectively; the control group received physiological saline (0.9% NaCl). Results of the studies showed that the levels of serum aspartate aminotransferase (AST) and alanine amino transferase (ALT) were significantly (P<0.05) increased in all the treatments compared with the control group. However, the serum levels of alkaline phosphatase, and lactate dehydrogenase were not significantly (P>0.05) affected. Only the group with 15 doses had their serum level of albumin significantly (P<0.05) increased. However, the results of histopathological studies showed that both the livers and hearts gave no pathological features for all the treatments. The results showed that prolong usage of this extract at 15-dose level could cause liver injury while the effect was mild at small dose levels (1-10). Though the average consumption of 150-180 mg/kg per day appears safe, the extract should be taken with caution bearing in mind that higher doses could affect the liver.


Publisher Kluwer Academic Publishers
Publication Type Journal
Publication Title Dynamics Of Phytate-zinc Balance Of Fungi Fermented Products (gari And Flour).
Publication Authors Oboh, G; Akindahunsi, A.A and Oshodi, A.A.
Year Published 2003
Abstract

Fungi fermented cassava products (gari and flour) were produced by fermenting cassava pulp with pure strains of some common saprophytes namely, Aspergillus flavus, Aspergillus niger, Rhizopus oryzae and Saccharomyces spp. (Baker’s yeast and palm wine yeast). The phytate contents were determined by titration of 2% HCl extract of the samples against standard FeCl3. Zn and Ca were determined using atomic absorption spectrophotometry. Calculation of [phytate]/[Zn], [Ca]/[phytate] and [Ca][phytate]/[Zn] molar ratios were used in predicting the effect of phytate on zinc bioavailability. The results of the studies revealed that there was a decrease in the phytate content of fungi fermented cassava products [flour (505.6 ± 4.2–748.5 ± 2.3 mg/100 g) and gari (373.5 ± 6.2–56.7 ± 5.2 mg/100 g)] when compared to naturally fermented cassava products [flour (705.1 ± 1.2 mg/100 g) and gari (633.9 ± 3.2 mg/100 g)] and unfermented cassava products [flour (874.4 ± 3.2 mg/100 g) and gari (662.8 ± 6.5 mg/100 g)]. The calculated [Ca][phytate]/[Zn] molar ratios for the products indicated that the fungi fermentation enhanced the bioavailability of zinc from the products. Moreover, values obtained in the present study were far below the critical value of 0.5 mol/kg. This indicates that fungi fermentation would bring about a decrease in the phytate content of cassava products (flour and gari) and consequently mprove the bioavailability of zinc from the products


Publisher A B Academic Publishers
Publication Type Journal
Publication Title Dynamics Of The Iodine-Goitrogen Balance In The Thyroid Status Of Catfish From Selected Fresh And Brackish Water Bodies.
Publication Authors Akindahunsi A.A., Salawu, S.O and Adu, O.C.
Year Published 2004
Abstract

The study was designed to investigate the dynamics of the goitrogen-iodine balance in Igbokoda brackish and Ogbese fresh water bodies in Ondo State, Nigeria. The parameters determined include iodine, thiocyanate, chloride, nitrate, calcium, magnesium hardness, calcium hardness, total hardness, and total coliform counts in water, sediment and/or fish (flesh and serum). The fish were subjected to a number of treatments (boiling with water or sodium chloride solution, frying or roasting) prior to analysis. The iodine level (Jlg I/1 OOg) in the flesh of fish varied with size, treatment and location; it increased with size, was reduced by some treatments (boiling with either water or NaCl solution), while frying and roasting did not affect the level. The least values were 93.45 (Ogbese fish boiled with NaCl solution) and 160.94 (Igbokoda fish boiled with either water or NaCl solution). On the whole, Igbokoda (160.94-314.08) had higher values than Ogbese (93.45-205.06). Serum iodine (Jlg IllOOmL) was equally higher in Igbokoda (5.00-9.00) compared to Ogbese (2.80-5.00). Only the large sized fish of Ogbese recorded a value of 5.00 Jlmol/L for thiocyanate while this parameter was not detected in other samples. The calculated I/goitrogen ratios (I/Ca, I/N03, I/Cl and IISCN) were higher for Igbokoda waterthan Ogbese water with the exception of I/Mg. This was equally reinforced by the total count of coliforms (goitrogenic), which was higher in Ogbese (3.0 x 103) than in Igbokoda (2.7 x 103). These results indicate that the Igbokoda brackish water body would be a better source of iodine than Ogbese fresh water body for the population dependent on them.


Publisher WFL Publisher
Publication Type Journal
Publication Title Nutrient Distribution In Wild And Cultivated Edible Mushroom, Pleurotus Sajor-caju.
Publication Authors Oyetayo, F.L and Akindahunsi A.A
Year Published 2004
Abstract

Two varieties of mushroom  Pleurotus sajor-caju,  obtained from the wild and cultivated (on shredded corncobs), were analysed (whole mushrooms and caps and stalks separately) on dry weight basis for their proximate, mineral and some antinutrient compositions. The caps of cultivated mushroom (Cc) accumulated higher concentrations of crude protein (26.34%) and ash (10.37%) than caps of the wild one (Wc), which had higher crude fat (3.90%) and crude fibre (16.32%) concentrations. The stalk of wild mushrooms (Ws) contained the highest concentration of crude fibre (26.14%). Of the nutritive elements analysed, potassium was the most dominant with concentration as high as 11.9 mg g-1 in the stalk of cultivated mushrooms (Cs). The caps of both varieties were found to contain zinc, phosphorus and magnesium in higher concentrations than their corresponding stalks. Iron and zinc in the cultivated mushroom (Cw) were more preponderant than in the wild one (Ww). Tannin concentrations (%TA) in cap and stalk of the wild variety were at an equal level (0.57) and lower than those in the cultivated one (0.76-0.99). The total cyanide concentration (mg/100g) in both varieties (0.00-0.02) was generally low. The calculated phytate/Zn and [Ca]:[phytate]/{Zn}molar ratios were below the  critical levels. 


Publisher WFL
Publication Type Journal
Publication Title Physicochemical Studies On African Oil Bean (Pentaclethra Macrophylla Benth.) Seed.
Publication Authors Akindahunsi A.A
Year Published 2004
Abstract
Some physicochemical parameters of the petroleum ether (40-60°C) extract as well as the effects of processing (salting, soaking before cooking and fermentation) on the proximate, antinutritional and mineral contents of African oil bean (Pentaclethra macrophyllaBenth.) seed were evaluated. The percentage oil yield was 53.6±0.7 on a dry matter basis and the colour was similar to that of groundnut oil (yellow) while the peroxide and saponification values were 1.2 mg/g and 32.4±0.1, respectively. The acid value, free fatty acid and specific gravity were 36.6±1.5 mg/g, 1.6, and 0.86 respectively. The refractive index and iodine value were 1.5 and 74.9. The oil gave no irritating odour. This coupled with the relative abundance and low cost holds a good promise with respect to commercial production of oils.
All the three treatments (salting, soaking before cooking and fermentation) significantly (P<0.05) decreased the protein content (%): (raw 41.7, soaking plus cooking 9.9, salting 10.5, fermentation 8.0). Conversely, the levels of fat (27.6, 48.5, 36.3, 51.5%), carbohydrate (3.9, 24.2, 28.2, 18.6%) and energy (312.5, 450.9, 440.5, 405.9 kcal/mol) for raw, soaking plus cooking, salting and fermentation samples, respectively, were increased. While soaking plus cooking (1.4%) and salting (1.2%) decreased the ash content, fermentation (7.8%) brought about an increase. While Zn level increased with the treatments, Mg, Na and K levels decreased. The treatments did not have any discernable trend in the antinutrients. Phytate level increased with treatments, cyanide level was reduced while there was no effect on the tannin level. There is therefore the need to develop methods for effective reduction of the antinutrients for optimal utilization of the abundant oil and the relatively high protein content of the African oil bean seed.

Publisher Elsevier
Publication Type Journal
Publication Title Vertebrate 2xRBD HnRNP Proteins: A Comparative Analysis Of Genome, MRNA And Protein Sequences.
Publication Authors Akindahunsi, A.A; Bandiera, A and Manzini, G
Year Published 2005
Abstract

hnRNP proteins are involved in many cell functions, primarily in pre-mRNA processing.We report here a comparative analysis of the genes of the 2xRBD members of the hnRNP family and of their expression products. Starting from the seven well characterized hnRNP members of human and murine origin (A0, A1, A2/B1, A3, AB, D and DL) and the three MuSashI-like proteins with related RBD tandems (MSI1, MSI2 and DAZAP1), we identified through BLAST search 12 homologous genes in the genome of Danio rerio and 10 in the genome of Takifugu rubripes, which can be divided into three subgroups, each with its highly conserved exon/intron structure, matching perfectly the exon/intron structures found in human and mouse genes. An exception is the gene of hnRNP A0, which is intronless consistently in all the four species. The analysis has been supported also at the level of cDNA and EST databases and extended in this respect to other vertebrate species, namely chicken, Xenopus laevis and Silurana tropicalis. PHYLIP 3.62 package (SEQBOOT, PROTDIST/DNADIST, NEIGHBOR, CONSENSE) was used for all the proteins and their CDSs and human RBDs I and II to infer relevant aspects of the phylogenesis of these proteins. Some clues to the evolution of introns in these genes have come from the analysis of their distribution in homologous genes of other eukaryotes, namely Ciona, Drosophila, Caenorhabditis, Saccharomyces and Arabidopsis.


Publisher Nil
Publication Type Journal
Publication Title Effect Of Processing On The Nutrients Of Sarotherodun Galilaeus And Claria Gariepinus Caught In Ogbese River , Ondo State, Southern Nigeria
Publication Authors Ojo, O.C, Salawu, S.O and Akindahunsi, A.A
Year Published 2005
Abstract

Nil


Publisher Springer-Verlag
Publication Type Journal
Publication Title Nutritive Value Of Fresh And Brackish Water Catfish As A Function Of Size And Processing Methods.
Publication Authors Salawu, S.O, Adu, O.C and Akindahunsi A.A
Year Published 2005
Abstract

Catfish (Clarias gariepinus) of different sizes (small, 30.33–30.83 cm and 191.67–195.00 g; medium, 36.67–37.50 cm and 275.00–303.33 g; large, 41.33–49.67 cm and 536.67–726.00 g) collected from brackish (Igbokoda) and fresh water (Ogbese) bodies was subjected to different treatments (fried, boiled with/without salt solution and roasted). The proximate (ash, fat and protein) and mineral (Ca, Zn, Fe, Mg, P and I) compositions of all the treatments were determined. The highest protein concentrations (on a dry weight basis percent) (Ogbese, 73.25; Igbokoda, 72.52) of fish samples were recorded by boiling with water, while the ones roasted recorded the lowest values of 57.51 and 58.33%, respectively. The iodine level (grams of I per 100 g) in the flesh of fish varied with size, treatment and location; it increased with size, was reduced by some treatments (boiling with either water or NaCl solution), while frying and roasting had the highest level compared with boiling. The lowest iodine concentrations were 93.45 (Ogbese fish boiled with NaCl solution) and 160.94 (Igbokoda fish boiled with either water or NaCl solution). On the whole, Igbokoda brackish water fish (160.94–314.08) had a higher iodine content than Ogbese fresh water fish (93.45–205.06) with the highest concentration in fried and roasted fish samples (205.06, 314.08) in Ogbese and Igbokoda water bodies, respectively. Higher mineral compositions were also found in the brackish water fish (for flesh and serum), and also increased with size and were reduced by some treatments (boiling and frying) in the flesh of fish samples. The fish (catfish) from the brackish water body appeared to be more nutritious than that from the fresh water body. The highest protein values were recorded by boiling with water, while frying and roasting gave the highest iodine value. Moreover, the study revealed that size is a factor to be considered by the population dependent on fish as a source of protein, iodine and other vital elements.


Publisher Academic Journals
Publication Type Journal
Publication Title Phytochemical Screening And Nutrient-Antinutrient Composition Of Selected Tropical Green Leafy Vegetables.
Publication Authors Akindahunsi, A. A.and Salawu S. O
Year Published 2005
Abstract

Levels of some nutrients and antinutrients in 14 commonly consumed tropical green leafy vegetables were evaluated and also screened for some phytochemicals. Saponin was present in all the vegetables with the exception of Hibiscus esculentus, Solanum macrocarpon and Piper guineese while only tannin was absent in Crassocephalum crepidioides, Talinum triangulare, Corchorous olitorius and Piper guineese. Crude protein, fat, fibre and ash recorded ranges from 20.59 to 38.18, 5.90 to 12.73, 6.20 to 7.20 and 8.00 to 25.49 %, respectively. Na (0.64 to 8.97 g/100 g) and P (1.34 to 3.34 g/100 g) recorded the highest values of minerals. The low levels of antinutrients (phytic acid, tannic acid and oxalate) coupled with high level of zinc biovalability indicates that the studied vegetables are good dietary supplements.


Publisher Nil
Publication Type Journal
Publication Title Antioxidant Indices Of Some Green Leafy Vegetables.
Publication Authors Akindahunsi A. A. and S. O. Salawu
Year Published 2005
Abstract

This study reports the antioxidant phytochemicals ascorbic acid, phytate and total phenols, free radical scavenging capacity and reducing properties of 14 green leafy vegetables commonly consumed in the southern part of Nigeria


Publisher Elsevier Ltd.
Publication Type Journal
Publication Title Nutrient And Antinutrient Distribution Of Edible Mushroom, Pleurotus Tuber-regium (Fries) Singer.
Publication Authors Akindahunsi, A.A. and Oyetayo, F.L
Year Published 2005
Abstract

Edible mushroom Pleurotus tuber-regium, separated into cap, stalk, and tuber, was analysed. The macronutrient profile (g/100 g) showed crude protein ranging from 4.1 to 13.8, with the highest concentration in the cap (13.8) than any of the other parts and total carbohydrates from 34.0 to 56.2, while the crude fat and ash contents were generally low. Potassium, the most abundant nutritive element was found to be the highest concentration (mg/g) in the stalk (3.3) while copper was found in trace amounts in all the parts. The total cyanide (mg/100 g), phytate (mg/100 g) and tannin (%TA) concentrations were all below levels considered harmful. Amino acids analysis show that the protein contained all essential amino acids while the calculated amino acids scores showed the Sulphur containing amino acids to be most limiting. The foregoing highlights the high nutritive values of the major parts of the edible mushroom, Pleurotus tuber-regium.


Publisher Elsevier
Publication Type Journal
Publication Title Anti-trichomonal, Biochemical And Toxicological Activities Of Methanolic Extract And Some Carbazole Alkaloids Isolated From The Leaves Of Murraya Koenigii Growing In Nigeria.
Publication Authors Adebajo, A.C., Ayoola, O.F., Iwalewa, E.O., Akindahunsi, A.A., Omisore, N.O.A., Adewunmi C.O. and Adenowo T.K
Year Published 2006
Abstract
The methanolic extract of Murraya koenigii leaf was screened for toxicological and biochemical effects on rats because of the folkloric uses as an anti-dysentery and anti-diabetes. The extract was moderately toxic (LD(50)=316.23 mg/kg body weight) to rats and had appreciable effect on the liver and kidney at higher doses leading to liver inflammation. It had little or no effect on haematology and relative organ weight of lungs, heart and spleen. Acute doses (500 mg/kg) reduced significantly serum globulin, albumin, urea, glucose, total protein, aspartate transaminase (AST), and increased cholesterol and alanine transaminase (ALT) indicating hepatic injury. However, chronic administration for 14 days gave a significant (p<0.05) reduction in the serum cholesterol, glucose, urea, bilirubin, ALT and AST showing that the plant has hypoglycaemic and hepatoprotective effects after prolonged use. The activity demonstrated by some of the isolated carbazole alkaloids and their derivatives against Trichomonas gallinae confirmed that the anti-trichomonal activity of the leaf may be due to its carbazole alkaloids. The order of activity was C(18)>C(23)>C(13). Girinimbine and girinimbilol with IC(50) values of 1.08 and 1.20 microg/ml were the most active. Acetylation of girinimbilol and mahanimbilol improved their activities to 0.60 and 1.08 microg/ml.

Publisher Academic Journals Inc, USA
Publication Type Journal
Publication Title Chemical Composition And In Vitro Antioxidant Activities Of Some Nigerian Vegetables.
Publication Authors Salawu, S.O., Akindahunsi, A.A and Comuzzo, P
Year Published 2006
Abstract
This study was designed to investigate the chemical composition of fourteen vegetables popularly consumed in the South Western part of Nigeria and also to evaluate some in vitro antioxidant activities of the selected vegetables. Ethanolic extracts of the vegetables were injected into GC-MS to investigate the presence of some chemical compounds and were also screened for their lipid peroxidation inhibitory potentials and free radical scavenging activities by two complementary. GC-MS studies showed that caffeic acid was present in all the vegetable samples with the exception of Celocia argentia and Talinum triangulare in addition to other compounds identified in some of the vegetables (phenylacetic acid, vanillic acid, genticic acid, protocatechuic acid, syringic acid, p-coumaric acid, garlic acid, ferulic acid, epicatechin and catechin. Lipid peroxidation inhibition studies revealed that lipid peroxide inhibition ranges from 3.59-69.47 (%) while conjugated diene inhibition ranges from 84.33-97.31 (%). Scavenging ability of the vegetables towards hydrogen peroxide ranges from 4.89±0.55-60.26±1.23 (%) while superoxide scavenging activities ranges from 75.62±1.42-97.01±1.32 (%). Total flavonoid mg mL-1
 and total antioxidant activity (mg mL -1) ranges from 0.12 to 3.08 and 0.13 to1.60, respectively. Results from conjugated diene formation and superoxide radical scavenging activity show that the evaluated vegetables exhibited a relatively high lipid peroxidation inhibitory potentials and free radical scavenging activity which could possibly be as a result of caffeic acid and other compounds present in the vegetables.

Publisher Academic Journals
Publication Type Journal
Publication Title Alchornea Cordifolia Extract Protects Wistar Albino Rats Against Acetaminophen-induced Liver Damage.
Publication Authors Olaleye M. T., Adegboye, O. O. Akindahunsi A. A.
Year Published 2006
Abstract

At the therapeutic doses, aetaminophen (N-acety1-p-aminophenol, paracetamol, AAP) is considered a safe drug; intake of toxic dosage could lead to liver disease. The hepatoxicity of AAP is mainly as a result of oxidative stress mediated by the metabolites of APP. The present work seeks to evaluate phytochemical constituents, antioxidative properties and hepatoprotective activities leaf extract of Alchornea cordifolia on acetaminophen-induced hepatoxicityin rats. Phytochemical analyses gave positive results for saponins, tannins and flavonoids. The antioxidative properties revealed total phenolic content of 0.22 mg/ml and reducing power 0.062 mg/ml as compared to vitamin E with a reducing power of 0.042 mg/ml. Oral administration of a single acetaminophen dose (2 g/kg) caused oxidative liver damage as determined by alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activities, cholesterol and bilirubin levels in the liver and the serum. The administration of 200-500 mg/kg A. cordifolia leaf extract for 2 weeks produced a significant dose-dependent curative/preventive effect on acetaminophen-nduced liver toxicity as reflected by the above biochemical markers. The protective effect compared favorably with putative antioxidant agents such as curcumin and Vitamin E. The results so far suggest that the hepatoprotective activity of this plant against acetaminophen-nduced liver damage is connected to its antioxidative properties


Publisher Academic Journals
Publication Type Journal
Publication Title Antioxidant Properties Of Myristica Fragrans Houtt And Its Effect On Selected Organs Of Albino Rats.
Publication Authors Olaleye, M.T, Akinmoladun, A.C and Akindahunsi A.A
Year Published 2006
Abstract

Aqueous extract of the seed of Myristica fragrans (nutmeg) was evaluated for its phytochemical constituents, antinutrients and antioxidant properties. Toxicological investigation was also carried out using six groups of Wistar albino rats. The treatment groups were administered varying doses (100-500 mg/kg body weight) of the extract for a period of 28 days. The animals were sacrificed and their livers, kidneys, hearts, spleen and testes harvested for histopathological studies. The results showed that alkaloids, saponins, anthraquinones, cardiac glycosides, flavonoids and phlobatanins were present while tannins were absent in the aqueous extract. The phytate content was 564.11 mg/100 g while the antioxidant indices of 100 mg/100 g, 44% and 0.6 were obtained for the ascorbic acid value, free radical scavenging activity and reducing power, respectively. The results of the histopathological studies showed pathological features of various degrees in the organs with severity corresponding to the concentration of extract. There was lymphoid depletion of the follicles in the spleen, degeneration of the germinal epithelial cells in the testes, bile duct proliferation and congestion of blood vessels in the liver, degeneration, necrosis with desquamation of tubular epithelial cells and congestion of renal blood vessels in the kidney and degeneration of myocardial fibres and myocardial necrosis in the heart in the treatment groups compared with the control. The present results suggest that nutmeg popularly consumed as food and for various medicinal purposes may contain some active principles with antioxidant properties. However, prolonged use at high doses (400-500 mg/kg) could be very toxic to the studied organs. 


Publisher Academic Journal
Publication Type Journal
Publication Title Studies Of Physiochemical And Microbial Properties Of Soils From Rainforest And Plantation In Ondo State, Nigeria
Publication Authors Oseni, O. A., Ekperigin, M. M., Akindahunsi, A. A. and Oboh, G
Year Published 2007
Abstract

Microbial population, and biomass as well as hydrolytic enzymes of soil that are responsible for biodegradation of various structural complexes of plant litters and dead organic compounds from Ondo State Aforestation project at Lisagbede (Oluwa forest reserve) were studied. Soil fungi and bacteria were isolated and identified from two sources namely Gmelina aborea plantation (planted for over 15 years) and an adjacent natural forest at different depth levels (0 - 10, 10 - 20 and 20 - 30 cm) by weighing 1.0 g of soil into 9.0 ml of sterilized water, shaken and diluted 1 in 1000, 0.5 ml each of this was poured onto separate sterilized Petri dish and overlaid with potato dextrose agar and nutrient agar, allowed to incubate at 27 and 32oC for five and one day respectively. The results of the study revealed that all the parameters showed slight increase in natural forest soil as compared to the G. aborea plantation soil. Substantive differences were obtained in soil microbial biomass for each sampling depth in the natural forest compared to the G. aborea plantation. While there were no much differences in the soil physicochemical properties of the forest and plantation. Bacteria and fungi isolates of the two locations and their enzymes activities were similar. Two fungi Aspegillus niger and Penicillium italicum isolated from the two locations produced hydrolytic enzyme activities of cellulase, _-amylase, _- amylase and protease which are degrading enzymes.


Publisher A B Academic Publishers
Publication Type Journal
Publication Title Coagulants Modulate The Antioxidant Properties & Hypocholesterolemic Effect Of Tofu (curdled Soymilk)
Publication Authors Oboh G, Ekperigin MM, Akindahunsi AA
Year Published 2007
Abstract

The recent increase in Soymilk and tofu (coagulated soymilk) consumption especially in Western Country is due to the recognition of the health benefits of soy foods; consumption of soybean would prevent heart diseases. In Nigeria Calcium salt, alum and steep water from pap production are usually used as coagulant in tofu production. The effect of those coagulants on the antioxidant properties of tofu and serum cholesterol, high-density lipoproteins (HDL) and low-density lipoproteins (LDL) level of albino rats fed tofu for 14 days is been assessed. The result of the study revealed that there was no significant difference (P > 0.05) in the tofu yield (17.6-18.3%), however steep water coagulated tofu had a significantly higher (P < 0.05) total phenol (12.0 g/kg) content, reducing power (0.6 OD700) and DPPH free radical scavenging ability (69.1%) than tofu produced using other coagulants. Furthermore, feeding albino rats with tofu and water ad libitum for 14 days caused a significant decrease (P < 0.05) in the serum cholesterol and low-density lipoproteins when compared with the control, while there was no significant difference (P > 0.05) in the average daily feed intake of the rats. Conversely, there was a significant increase (P < 0.05) in the serum high-density lipoproteins when compared with the control. However, rats fed steep water coagulated tofu had the lowest serum level of cholesterol and LDL level followed by those fed CaCl2 and alum coagulated tofu respectively, while those fed with calcium chloride coagulated tofu had the highest serum HDL level, and closely followed by those fed steep water coagulated tofu. It was therefore concluded that of all the coagulant, steep water appeared to be the most promising coagulant with regard to the production of tofu with higher antioxidant and hypocholesterolemic effect.


Publisher Nil
Publication Type Journal
Publication Title Protective Effect Of Some Tropical Vegetables Against CCl4-induced Hepatic Damage
Publication Authors Salawu SO, Akindahunsi AA
Year Published 2007
Abstract

Nil


Publisher Nil
Publication Type Journal
Publication Title Nutritional Quality Of Sandbox Tree (Hura Crepitans Linn.)
Publication Authors Fowomola MA, Akindahunsi AA
Year Published 2007
Abstract
Antinutrient, proximate, mineral, fatty acid, vitamin, and amino acid analyses of sandbox tree (Hura crepitans) seeds were carried out. The results of antinutrient analysis showed that H. crepitans seed contains alkaloid (5.0 +/- 0.2 mg/100 g), tannins (5.0 +/- 0.3 mg/100 g), phytate (53.0 +/- 6.0 mg/100 g), cardiac glycoside (1890.0 +/- 1.5 mg/100 g), and saponin (2.2 +/- 0.1 mg/100 g). Its trypsin inhibitor activity was found to be 30.27 +/- 1.86 TIU/mg of protein. The results of proximate analysis showed that H. crepitans seed is very rich in crude protein (25.16 +/- 0.22%), crude oil (51.43 +/- 0.22%), and energy content (2,621.891 +/- 6.357 kJ/100 g). H. crepitans seed also contains 1.85 ppm Na, 3.4 ppm K, 0.088 ppm Ca, and trace amounts of Mg, Fe, and Zn. The results also showed that H. crepitans oil contains 20.12% oleic acid, followed by stearic acid (3.0%), while linoleic acid is present at the lowest level (0.03%) among the other acids. The Hura oil has a high saponification value (127.16 +/- 0.18 mg/g) and low acid value (3.56 +/- 0.16 mg/g). The results also showed that the average molecular weight of glycerides is higher in the oil as reflected by the ester value (123.6 +/- 0.73 mg/g). The iodine value of Hura oil was found to be 65.62 +/- 0.73%. A low peroxide value (6.6 +/- 0.2 mg/g) was observed in Hura oil. The results showed that H. crepitans seed contains 328.1 IU of vitamin A/100 g, 0.398 mg of vitamin E/100 g, and 0.26 mg of vitamin K/100 g. The results also showed that H. crepitans seed is very rich in glutamate (14.41 g/100 g of protein) and deficient in cysteine (0.78 g/100 g of protein). Among the essential amino acids, arginine has the highest value (5.97 g/100 g of protein). This is followed by leucine, at 4.16 g/100 g of protein. Therefore, H. crepitans seed is a nutritionally promising seed.

Publisher A B Academic Publishers
Publication Type Journal
Publication Title Chemical Profile And Amino Acids Composition Of Edible Mushrooms Pleurotus Sajor-Caju.
Publication Authors Oyetayo, F.L., Akindahunsi A.A and Oyetayo V.O
Year Published 2007
Abstract
Two varieties of Pleurotus sajor-caju, obtained from the wild and cultivated (on shredded corncobs) were separated into cap and stalk and analysed on dry weight basis. The proximate composition (g/100 g) showed that both varieties were highly concentrated in crude protein. The cultivated cap (Cc) was found to accumulate higher concentration of crude protein (26.34%) and ash (10.37%) than the wild cap (Wc), which had higher crude fat (3.90%) and crude fibre (16.32%) concentrations while the wild stalk (Ws) contained the highest concentration of crude fibre (26.14%). Amino acid analysis showed the mushroom protein to be a rich source of nutritionally useful essential amino acids with leucine as the most abundant occurring in highest concentration (mg/g) in the Cc (64.8 +/- 0.24). Methionine and cysteine concentrations were low as confirmed by their chemical scores (0.29-0.59). There was a significant difference (P<0.05) in the distribution of amino acids in the mushroom varieties and parts. The total essential amino acid (TEAA) in the cultivated range between 42.91-43.69% while in the wild it was between 42.90-43.73 % of the total amino acid content. The amino acid concentration was a function of mushroom variety and part selected.

Publisher Nil
Publication Type Journal
Publication Title Antioxidant Indices Of Some Tropical Vegetables And The Effects Of Some Hepatic Enzyme Markers In Garlic-fed Albino Rats.
Publication Authors Akindahunsi, A.A
Year Published 2007
Abstract

Nil


Publisher Academic Journals
Publication Type Journal
Publication Title Studies Of Physiochemical And Microbial Properties Of Soils From Rainforest And Plantation In Ondo State, Nigeria.
Publication Authors Oseni, O. A., Ekperigin, M. M., Akindahunsi, A. A. and Oboh, G
Year Published 2007
Abstract
Microbial population, and biomass as well as hydrolytic enzymes of soil that are responsible for biodegradation of various structural complexes of plant litters and dead organic compounds from Ondo State Aforestation project at Lisagbede (Oluwa forest reserve) were studied. Soil fungi and bacteria were isolated and identified from two sources namely Gmelina aborea plantation (planted for over 15 years) and an adjacent natural forest at different depth levels (0 - 10, 10 - 20 and 20 - 30 cm) by weighing 1.0 g of soil into 9.0 ml of sterilized water, shaken and diluted 1 in 1000, 0.5 ml each of this was poured onto separate sterilized Petri dish and overlaid with potato dextrose agar and nutrient agar, allowed to incubate at 27 and 32oC for five and one day respectively. The results of the study revealed that all the parameters showed slight increase in natural forest soil as compared to the G. aborea plantation soil. Substantive differences were obtained in soil microbial biomass for each sampling depth in the natural forest compared to theG. aborea plantation. While there were no much differences in the soil physicochemical properties of the forest and plantation. Bacteria and fungi isolates of the two locations and their enzymes activities were similar. Two fungi Aspegillus niger and Penicillium italicum isolated from the two locations produced hydrolytic enzyme activities of cellulase, α-amylase, β- amylase and protease which are degrading enzymes
 

Publisher Nil
Publication Type Journal
Publication Title Hepatoprotective Activity Of Leaf- Extract Of Calotropis Procera Against Acetaminophen- And Hepatitis – Induced Liver Damage In Rats.
Publication Authors Olaleye, M.T, Akindahunsi, A.A. Ewemade, E
Year Published 2008
Abstract

Nil


Publisher Nil
Publication Type Journal
Publication Title Phenolic Profiles Of Four Processed Tropical Green Leafy Vegetables Commonly Used As Food.
Publication Authors Salawu, S.O, Giaccherini C, Innocenti M, Akindahunsi, A. A and Mulinacci, N
Year Published 2008
Abstract

The phenolic profiles are presented of four tropical green leafy vegetables (Ocimum gratissimum, Vernonia amygdalina, Corchorus olitorius and Manihot utilissima) commonly used as food, after application of traditional treatments, such as boiling and abrasion. The HPLC/DAD/MS technique was mainly used to carry out this study. Preliminary evaluation of the antioxidant properties of the vegetables was also performed using the DPPH in vitro test. For the first time, seasonal variations in the phenolic content of the four investigated vegetables were highlighted. Of the four plants, all showed only quantitative differences, except for Ocimum graticimum, in which cichoric acid, previously detected as one of the main constituents of this vegetable collected in November (dry season), was absent in the sample harvested in March. The phenolic constituents are chemically unmodified after a strong heating process, such as the traditional blanching (about 15 minutes) applied by Nigerian people prior to consuming these vegetables. Nevertheless, these typical preparations showed a consistent decrease in the total phenolic compounds with respect to the raw material, particularly for Corchorus olitorius (from 42.3 to 5.56 mg/g dried leaves) and Vernonia amygdalina (from 40.2 to 4.4 mg/g dried leaves). As expected, when the blanching treatment is reduced to a few minutes, as for Manihot utilissima leaves, the cooked vegetable maintained almost unaltered its original phenolic content (around 10 mg/g dried leaves). The unique exception is the blanched Ocimum gratissimum sample that showed a consistent increment of the total phenols, particularly of rosmarinic acid (from 6.1 to 29.8 mg/g dried leaves) with respect to the unprocessed vegetable.


Publisher Taylor & Francis
Publication Type Journal
Publication Title Fermentation Changes The Nutritive Value, Polyphenol Distribution And Antioxidant Properties Of Parkia Biglobosa Seeds (African Locust Beans).
Publication Authors Oboh G, Alabi K. B. and Akindahunsi, A. A.
Year Published 2008
Abstract

This present study was carried out to investigate the effect of natural fermentation on the nutritive value (proximate composition), polyphenolic distribution, and antioxidant properties of African locust beans (Parkia biglobosai). African locust bean (ALB) seeds were boiled for 12 h, dehulled and subjected to natural solid substrate fermentation without any inoculation for 4 days. The proximate composition of the fermented and unfermented locust bean seeds was subsequently determined. Thereafter, the polyphenol distribution, reducing power, DPPH free-radical scavenging antioxidant activity, and inhibition of lipid peroxidation were determined for soluble free and bound polyphenol extracts. The results of the study revealed that fermentation caused a significant increase (P < 0.05) in the ALB protein content with 18.3% in unfermented and 36% in fermented. The fat content in unfermented was 13.2% versus 26.8% in fermented, while there was no significant changes (P > 0.05) in ash, crude fiber, or moisture content. However, there was a significant decrease (P < 0.05) in the carbohydrate content. The total phenolic content, reducing ability and free-radical scavenging ability were high in both the fermented and unfermented ALB. Fermentation caused a significant increase (P < 0.05) in the free phenolic content of the ALB and a significant decrease (P < 0.05) in the bound phenolic content of ALB. The free-phenolic fraction from both fermented and unfermented ALB had high reducing power, free radical scavenging ability, and inhibition of lipid peroxidation using cow brain tissues. Free phenolic extract from the fermented ALB had a significantly higher (P < 0.05) antioxidant activity than that of unfermented ALB. Results suggest that fermentation will increase the nutritive values, free phenolic and antioxidant activity of the African locust bean, making it a potential functional food in addition to its traditional role of dietary protein source.


Publisher Nil
Publication Type Journal
Publication Title Phenolic Profiles Of Four Processed Tropical Green Leafy Vegetables Commonly Used As Food.
Publication Authors Sule Ola Salawu, Marzia Innocenti, Catia Giaccherini, Afolabi Akintunde Akindahunsi and Nadia Mulinacci
Year Published 2008
Abstract

Nil


Publisher Elsevier
Publication Type Journal
Publication Title HPLC/DAD/MS Characterisation And Analysis Of Flavonoids And Cynnamoil Derivatives In Four Nigerian Green-leafy Vegetables
Publication Authors Salawu, S.O, Giaccherini C, Innocenti M, Vincieri, F. F, Akindahunsi, A. A and Mulinacci N
Year Published 2009
Abstract

The present study sought to carry out a screening of the phenolic fraction of four Nigerian plants, Ocimum gratissimum L. (Og), Vernonia amygdalina L. (Va), Corchorus olitorius L., (Co) and Manihot utilissima Pohl. (Mu) consumed as food at least once daily by people in southwestern Nigeria and also used for medicinal purposes by local populations. HPLC/DAD and HPLC/ESI/MS analyses were applied as the most suitable techniques to investigate the phenolic content of the dried leaves. This screening allowed detection of up to 17 constituents in Va, five phenols in Mu, eight and 11 different metabolites in Co and Og, respectively. Some compounds have been detected for the first time in these vegetables: cichoric acid in Og, amentoflavone in Mu and several dicaffeoyl compounds in Va and Co. The study of the decoction demonstrated that almost all the phenolic constituents are stable even after a strong heating process such as boiling, as usually applied by Nigerian people prior to the consumption of these vegetables.


Publisher SAGE
Publication Type Journal
Publication Title Change In The Polyphenol Distribution And Antioxidant Activity During Fermentation Of Some Underutilized Legumes.
Publication Authors Oboh G, Ademiluyi A. O and Akindahunsi A. A.
Year Published 2009
Abstract
The effect of fermentation on the polyphenol distribution and antioxidant activity of four underutilized legumes [Cajanus cajan L. Millsp (Pigeon pea), Vigna subterranea L. Verdc (Bambara groundnut), Sphenostylis stenocarpa Harms (African yam bean), and Phaseolus vulgaris L. (Kidney bean)] were investigated. The beans were cooked (12 h), soaked in boiled water (12 h), dehulled, and then cooked again (2 h). The cotyledons were drained, wrapped in jute sacks and left to ferment at 37°C (4 days) to produce condiments. The distribution of free and bound phenolic compounds in the fermented and unfermented beans was determined; thereafter the free radical scavenging ability, reducing power, and the ability of the free and bound phenolic compounds of the fermented and unfermented beans to inhibit lipid peroxidation were determined. The results of the study revealed that fermentation caused a significant increase (p<0.05) in the free soluble phenol content of the legumes, while there was a significant decrease (p<0.05) in the bound phenol content of the legumes. Free soluble phenol from both the fermented and unfermented legumes had a significantly (p<0.05) higher reducing power (except C. cajan), free radical scavenging ability (except C. cajan) and inhibition of lipid peroxidation than bound phenolic extract. However, free soluble phenolic compounds from the fermented beans had a significantly higher (p<0.05) reducing power, free radical scavenging ability, and inhibition of lipid peroxidation than free soluble phenols from unfermented beans. Hence, it was concluded that, fermentation could increase the free soluble phenolic content of the underutilized legumes tested and consequently enhance the antioxidant activities of the legumes; fermented V. subterranea was found to be the most promising condiment with antioxidant activity.

Publisher NISEB
Publication Type Journal
Publication Title R-α–Lipoic Acid And Acetyl-L-carnitine Optimal Combinations In MPP+- Induced Cellular Model Of Parkinson’s Disease
Publication Authors Akindahunsi A. A.
Year Published 2009
Abstract

Mitochondrial insufficiency and oxidative damage contribute to the etiopathology of Parkinson’s disease (PD). However, there is a dearth of information on the protective activities against PD of mitochondrial nutrients, safe for coenzyme Q10. In the present study, the PD protective effects of two mitochondrial nutrients, R-_–lipoic acid (LA) and acetyl-Lcarnitine (ALC), as well as their combinations using 1-methyl-4-phenylpyridinium ion (MPP+)-treated SKN-MC human neuroblastoma cells as a model of PD was examined. Pretreatment of cells with LA (1-100 μM), ALC (1-100 μM) or LA-ALC (1:10; 10:100;100:100 μM) combinations showed protective effects against MPP+-induced toxicity of cells. The best concentrations were LA-ALC (1:10 μM) combination, LA (10 μM) and ALC (100 μM) in that order, thus indicating a synergy by the mitochondrial nutrients. This could be a promising strategy in combating PD and other neurodegenerative disorders.


Publisher Elsevier Ltd.
Publication Type Journal
Publication Title Antioxidant Activity And Hepatoprotective Property Of Leaf Extracts Of Boerhaavia Diffusa Linn Against Acetaminophen-induced Liver Damage In Rats.
Publication Authors Olaleye MT, Akinmoladun AC, Ogunboye AA, Akindahunsi AA
Year Published 2010
Abstract

Extracts of Boerhaavia diffusa leaves were evaluated for antioxidant and hepatoprotective properties in the acetaminophen-induced liver damage model. Antioxidative evaluation of ethanolic extract gave total phenolic content, total flavonoid content, vitamin C content and vitamin E content and the levels of selenium and zinc as 6.6+/-0.2mg/g tannic acid equivalent, 0.092+/-0.003 mg/g quercetin equivalent, 0.21+/-0.03 mg/g, 0.054+/-0.002 mg/g, 0.52+/-0.05 ppm and 9.28+/-0.16 ppm, respectively. The DPPH scavenging capacity and the reductive potential were 78.32+/-2.41% and 0.65+/-0.02 mg/g ascorbic acid, respectively. Pretreatment with aqueous and ethanolic extracts decreased the activities of alkaline phosphatase, lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, and the level of bilirubin in the serum that were elevated by acetaminophen. The two extracts also ameliorated the elevation in the activities of the enzymes in the liver. Acetaminophen intoxication led to reduction in serum and liver albumin levels which were not significantly increased by pretreatment with the extracts. The extracts also protected against acetaminophen induced lipid peroxidation. These results indicated that leaf extracts from B. diffusa possess hepatoprotective property against acetaminophen-induced liver damage which may be mediated through augmentation of antioxidant defenses.


Publisher Academic Journal
Publication Type Journal
Publication Title Cellular Antioxidant Activities And Cytotoxic Properties Of Ethanolic Extracts Of Four Tropical Green Leafy Vegetables.
Publication Authors Salawu, S.O, Akindahunsi, A.A, Sanni, D.M, Decorti G, Cvorovic J, Tramer, F., Passamonti and Mulinacci N
Year Published 2011
Abstract

The present investigation sought to evaluate cellular antioxidant activities of some green leafy vegetables: Vernonia amygdalina, Asteraceae (Va,); Manihot utilissima Pohl., Euphoriaceae (Mu);Corchorous olitorius L., Malvaceae (Co) and Occimum gratissimum L., Lamiaceae (Og) against peroxyl radical-induced oxidation in HepG2 cells and also evaluate their cytotoxic properties against drug sensitive and drug resistant Lovo and Lovo/Adr cancer cell lines respectively. HPLC/DAD/MS investigation of the ethanolic extract of the vegetal matter revealed the presence of 4, 5, 6, and 7 phenolic compounds in Mu, Co, Og and Va respectively. The cellular antioxidant activity was tested with different concentrations (0.1, 0.25, 0.5 and 1.00 mg/ml dried weight) of the extracts. At a concentration of 1.00 mg/ml, the quenching of peroxyl radical in HepG2 cells as shown by the fluorescence units revealed that Og has the best inhibition capacity, followed by Co, Va respectively and with the least inhibition capacity recorded for Mu. However, pro-oxidant activities were observed at lower concentrations of Va (0.1 mg/ml) and Mu (0.1, 0.25 and 0.5 mg/ml). Cytotoxic investigation revealed that the extracts were active against both cell lines with EC50 (μg/ml) ranging from 1.3 to 2.6 for Lovo and 1.5 to 2.7 for Lovo/Adr respectively, and that Va was the most active, while the least activity was recorded for Mu and Co for Lovo and Lovo Adr respectively. The observed cytotoxic properties and the elicited antioxidant activities would be due to the phytochemical constituents among which could be the rich content of flavonoids and cynammoyl derivatives as revealed by the chromatographic investigations.


Publisher Nil
Publication Type Journal
Publication Title Effects Of Fire On Physico-chemical Properties And Proteolytic Enzyme Activities Of Soil From Teak (Tectona Grandis L.F) Plantations
Publication Authors Oseni O. A., Ekperigin, M. M., Oboh G., Akindahunsi A.A. and Adekunle V.A. J.
Year Published 2010
Abstract

Nil


Publisher Nil
Publication Type Journal
Publication Title Effect Of Roasting On The Nutritive Value And Antioxidant Properties Of Maize.
Publication Authors Oboh G, Ademiluyi A.O. and Akindahunsi A. A.
Year Published 2010
Abstract

Nil


Publisher Academic Journals
Publication Type Journal
Publication Title Changes In The Total Phenol Content And Antioxidant Properties Of Pepperfruit (Dennettia Tripetala) With Ripening.
Publication Authors Adedayo, B. C., Oboh, G. and Akindahunsi A. A
Year Published 2010
Abstract

Pepperfruit (Dennettia tripetala G. Baker (Annonaceae) is a well-known Nigerian spicy medicinal plant normally used in folk medicine to cure fever, cough, toothache, as well as a stimulant and in the preparation of some special dishes for pregnant and postpartum women. The present study sought to assess the antioxidant properties of the aqueous extract of ripe (RPF) and unripe pepperfruit (UPF) extract. The aqueous extract of the pepperfruits were prepared, and their antioxidant phytoconstituents (total phenol, total flavonoid and ascorbic acid) and activities (Fe 2+ chelation ability, OH* scavenging ability, reducing power, DPPH and ABTS radical scavenging abilities) were subsequently determined. The total phenol (1.4 mg/g) content of RPF was significantly higher (P < 0.05) than that of UPF (1.0 mg/g), while there was no significant difference in the vitamin C and total flavonoid content. Conversely, UPF had higher antioxidant activities, as typified by higher reducing power, DPPH*, ABTS* and OH* scavenging abilities, and Fe chelating ability. Therefore, the physiological changes that accompany ripening of pepperfruit that brings about changes in pigment would increase the total phenol content, but decreases the antioxidant properties of pepperfruit.


Publisher Academic Journals
Publication Type Journal
Publication Title Biochemical, Haematological And Histopathological Studies Of Extract Of Ageratum Conyzoides L. In Sprague Dawley Rats.
Publication Authors Adebayo, A. H; Zeng, G. Z; Fan, J. T; Ji, C. J; He, W. J; Xu, J. J; Zhang, Y. M; Akindahunsi, A. A; Kela, R and Tan, N. H
Year Published 2010
Abstract

This study was conducted to evaluate the safety potential of the leaf extract ofAgeratum conyzoides Linnaeus in Sprague Dawley (SD) rats using biochemical, haematological and histological indices of toxicity. Four groups of seven male SD rats per group were used for the study. To group A was administered 0.25% CMC-Na/ kg body weight and was used as the control group, while groups B, C and D were respectively administered with 500, 1000 and 1500 mg/kg body weight of the ethanolic leaf extract of A. conyzoides by gastric intubation for 14 days. Animals were subsequently anaesthetized, blood samples were collected for biochemical and haematological assays; organs were isolated and weighed, while the liver, kidney and spleen were processed for histopathological studies. Aspartate amino transferase, lactate dehydrogenase, creatine kinase and alkaline phosphatase were significantly (p < 0.05) reduced in the groups treated with 1000 and 1500 mg/kg body weight of the extract. Furthermore, there was a significant (p < 0.05) elevation in white blood cell count, mean platelet volume and % platelet distribution width. Histopathological studies indicated various degrees of hepatocellular necrosis in all the treated groups accompanied by significant increases in the weight of liver and spleen. The results showed that the ethanolic leaf extract of A. conyzoides significantly alters the biomarkers of cardiac and skeletal muscle disorders, and higher doses could induce liver cell injury.


Publisher Medknow
Publication Type Journal
Publication Title Anticancer And Antiradical Scavenging Activity Of Ageratum Conyzoides L. (Asteraceae).
Publication Authors Adebayo, A.H., Tan, N.H. Akindahunsi, A.A. Zeng, G.Z. Zhang, Y.M.
Year Published 2010
Abstract

Ageratum conyzoides has been used in folklore for the treatment of a wide range of diseases. In the present investigation, the in vitro activity of ethanol, petroleum ether, ethylacetate, butanol, and water extracts of A. conyzoides were screened in some cancer cell lines using the sulphorhodamine B (SRB) assay. These cell lines include: Human non-small cell lung carcinoma (A-549), human colon adenocarcinoma (HT-29), human gastric carcinoma (SGC-7901), human golima (U-251), human breast carcinoma (MDA-MB-231), human prostate carcinoma (DU-145), human hepatic carcinoma (BEL-7402), and mouse leukemia (P-388) cancer cell lines. Furthermore, kaempferol was isolated from the ethylacetate extract and the structure was elucidated by nuclear magnetic resonance (NMR) and mass spectroscopy. The effect of DPPH antiradical activity on the extracts and kaempferol was also determined. The results showed that ethylacetate extract exhibited the highest cytotoxic activity on A-549 and P-388 cancer cells with IC50 values of 0.68 and 0.0003 g/ml, respectively. Kaempferol isolated from the ethylacetate extract of A. conyzoides rapidly scavenged DPPH at a concentration of 130.07 ± 17.36 g/ kg. The result therefore showed that A. conyzoides possessed anticancer and antiradical properties.


Publisher Academic Journal
Publication Type Journal
Publication Title Toxicological Evaluation Of Precocene II Isolated From Ageratum Conyzoides L. (Asteraceae) In Sprague Dawley Rats.
Publication Authors Abiodun Humphrey Adebayo, Guang Zhi Zeng, Yu Mei Zhang, Chang Jiu Ji, Afolabi Akintunde Akindahunsi and Ning Hua Tan,
Year Published 2010
Abstract
Precocene II (6,7-dimethoxy-2,2-dimethyl-2-chromene) was the main constituent isolated from Ageratum conyzoides L. and reportedly possessed antifungal activity. The study investigated the isolation, purification and toxicological effects of precocene II from A. conyzoides in Sprague Dawley rats. Precocene II was isolated from the petroleum ether fraction of the plant and the structure was determined by1H-,13C-,DEPT-NMR and MS spectral techniques. Three groups of eight rats per group were used for the study. While groups B and C were respectively administered with 25 and 50 mg/kg of precocene II in 0.25% CMC-Na for 11 days by gastric intubation, group A was administered with 0.25% CMC-Na and served as the control group. After the last treatment, animals were fasted overnight and on the 12th day, they were injected intravenously with 0.2 ml/kg body weight of phenobarbital. Animals were subsequently dissected from the abdominal region; blood was collected from the pulmonary vein into EDTA anti-coagulated and non anti-coagulated tubes.  The liver, kidney and spleen tissues were extracted into separate bottles for histopathological examinations. Results from hematological study indicated that the white blood cell (WBC), red blood cell (RBC), plateletcrit (PCT) and mean corpuscular hemoglobin count (MCHC) were significantly higher across the treated groups. Biochemical result showed that serum glucose level was significantly reduced in the treated groups. No apparent damage was noticed in the liver, kidney and spleen tissues. The result therefore suggests that precocene II possesses hypoglycemic property and could alter some hematopoietic elements but was not toxic to the liver, kidney and spleen tissues.

Publisher Academic Journal
Publication Type Journal
Publication Title Biochemical, Haematological And Histopathological Studies Of Ethanolic Leaf Extract Of Ageratum Conyzoides In Sprague Dawley Rats.
Publication Authors Abiodun-Humphrey Adebayo, Guang-Zhi Zeng, Yu-Mei Zhang, Jun-Ting Fan, Chang Jiu Ji, Akintunde-Afolabi Akindahunsi, Roseline Kela, Ning-Hua Tan
Year Published 2010
Abstract
This study was conducted to evaluate the safety potential of the leaf extract ofAgeratum conyzoides Linnaeus in Sprague Dawley (SD) rats using biochemical, haematological and histological indices of toxicity. Four groups of seven male SD rats per group were used for the study. To group A was administered 0.25% CMC-Na/ kg body weight and was used as the control group, while groups B, C and D were respectively administered with 500, 1000 and 1500 mg/kg body weight of the ethanolic leaf extract of A. conyzoides by gastric intubation for 14 days. Animals were subsequently anaesthetized, blood samples were collected for biochemical and haematological assays; organs were isolated and weighed, while the liver, kidney and spleen were processed for histopathological studies. Aspartate amino transferase, lactate dehydrogenase, creatine kinase and alkaline phosphatase were significantly (p < 0.05) reduced in the groups treated with 1000 and 1500 mg/kg body weight of the extract. Furthermore, there was a significant (p < 0.05) elevation in white blood cell count, mean platelet volume and % platelet distribution width. Histopathological studies indicated various degrees of hepatocellular necrosis in all the treated groups accompanied by significant increases in the weight of liver and spleen. The results showed that the ethanolic leaf extract of A. conyzoides significantly alters the biomarkers of cardiac and skeletal muscle disorders, and higher doses could induce liver cell injury.

Publisher Nil
Publication Type Journal
Publication Title Comparative Studies On Antioxidant Properties Of Some Tropical Nigerian Yam Varieties (Dioscorea Spp.)
Publication Authors Christiana B. Adedayo, Ganiyu Oboh, Adedayo O. Ademiluyi and Afolabi A. Akindahunsi
Year Published 2011
Abstract

The purpose of the present study was to assess and compare the antioxidant properties of the aqueous extracts of 5 popularly consumed Nigerian yams [Dioscorea dumetorum (white and yellow), Dioscorea cayenesis, Dioscorea rotundata and Dioscorea alata]. The aqueous extracts were prepared and their antioxidant phytoconstituents (total phenols and total flavonoids) and activities (Fe2+ chelating ability, OH􀀡 scavenging ability, reducing power, DPPH and ABTS radical scavenging abilities) were subsequently determined. The total phenol contents of the yams ranged from 0.88 - 2.45mg/g and total flavonoids from 0.11 - 0.81mg/g. However, the aqueous extract of Dioscorea dumetorum (white) had the highest total phenol content while Dioscorea rotundata had the highest total flavonoid content. Nevertheless, Dioscorea dumetorum (yellow) had the highest antioxidant activities as typified by higher reducing power, DPPH and ABTS􀀡 radical scavenging ability, and Fe2+ chelating ability (except the OH􀀡 scavenging ability).


Publisher Academic Journals
Publication Type Journal
Publication Title Some Phytochemical Properties And Effect Of Fermentation On The Seed Of Jatropha Curcuas L.
Publication Authors Oseni, O. A. and Akindahunsi, A. A
Year Published 2011
Abstract
The present study was undertaken to investigate the effects of microbial fermentation of Jatropha curcas L. seed (Barbados nut or Physic nut) or (Lapalapa) in Yoruba Language which are nutritionally underutilized. The study also sought to screen for some phytochemicals, determine the proximate, anti nutrient and some antioxidant properties of the fermented and non-fermented seed powder of Jatropha curcas L. to establish the multi-purpose usefulness of the seed, as well as determine the physicochemical properties of the extracted oil. The results showed that fermentation caused slight decrease in the carbohydrate, fat and crude fiber contents but enhanced the protein and ash contents of the seed while the antinutrient contents (tannins, phytate, oxalate and trypsin inhibitor) was observed to decrease as a result of fermentation. The antioxidant properties also witnessed a decrease in percentage 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical inhibition, amount of total phenolic compounds and super oxide anion radical inhibition as a result of fermentation. The phytochemical screening of methanolic and ethyl acetate extracts showed positive results for some secondary metabolites like tannins, glycosides, steroids, saponins and alkaloids in the unfermented seeds but slightly positive for the fermented seeds. The physicochemical analyses of the oil such as acid value, iodine value, saponification value, peroxide value and free fatty acid of unfermented seed appear to be higher than the fermented seed. The results of the analyses showed that the seed of Jatropha curcas possessed good antioxidant potentials and if fermented could enhance its nutritional qualities and reduce its anti-nutrients properties.

Publisher Academic Journals
Publication Type Journal
Publication Title Antimicrobial Activities Of Phenolic Containing Extracts Of Some Tropical Vegetables.
Publication Authors Salawu, S.O, Ogundare, A.O, Ola-Salawu, B.B, Akindahunsi, A.A
Year Published 2011
Abstract

The present study sought to investigate the antimicrobial properties of phenolic containing extracts of Vernonia amygdalina (Va), Ocimum gratissimum (Og) andManihot utilissima (Mu). Phenolic compound was characterized with the aid of a reversed phase HPLC/DAD/MS, and the antimicrobial activities of the extracts was assessed using agar-well diffusion method against some microorganisms, namely;Bacillus cereus, Escherichia coli, Salmonella spp, Pseudomonas aeruginosa, Staphylococcus aereus, Shigella spp, Enterobacter, Clostridium sporogenes, Bacillus subtilis and Proteus vulgaris. Ten, eight and four phenolic compounds were identified inVa, Og, and Mu respectively. The major phenolic compounds identified in Va were, Luteolin-7-O-glucoside, luteolin-7-O-glucuronide, luteolin and 1,5 dicaffeoyl quinic acid; nevadensin, vicenin-2, cichoric acid and rosmarinic acid in Og, while the major polyphenol in Mu were rutin  and Kaempferol 3-O-rutinoside. The antimicrobial investigation showed that M. utilissima is active against only B. cereus of the entire tested organism. V. amygdalina is active against B. cereusS. aereus and Shigella spp, while O. gratissimum is active against B. cereusS. aereus and Shigella sppThe results obtained in the present investigation showed that the use of the vegetable materials as nutraceuticals may reduce the risk of microbial infections, which may partly be due to their phenolic composition.


Publisher Nil
Publication Type Journal
Publication Title A New Chromene Isolated From Ageratum Conyzoides.
Publication Authors Adebayo, A. H; Ji, C. J; Zhang, Y. M; He, W. J; Zeng, G. Z; Han, H. J; Xu, J. J; Akindahunsi, A. A; and Tan, N. H
Year Published 2011
Abstract

Nil


Publisher Nil
Publication Type Journal
Publication Title Interaction Of Aqueous Extracts Of Two Varieties Of Yam Tubers Dioscorea Spp On Some Key Enzymes Linked To Type 2 Diabetes In Vitro.
Publication Authors Adedayo B.C., Ademiluyi A.O., Oboh, G. and Akindahunsi A.A.
Year Published 2012
Abstract

Nil


Publisher Nil
Publication Type Journal
Publication Title Effect Of Boiling And Drying Methods On The Nutrient Composition And Antioxidant Properties Of Sweet Potato (Ipomea Batatas) Flour.
Publication Authors Salawu, S.O., Akindahunsi, A.A., C.T. Ojo., Fashuyi, A, Olukemi, B.E, B. B. Ola-Salawu
Year Published 2012
Abstract

The purpose of this study was to investigate the effect of boiling and drying methods (oven and sun drying) on the nutrient composition and antioxidant potential of sweet potato flour. The result of the proximate composition showed that boiling and subsequent drying (oven and sun drying) do not affect the protein, carbohydrate, fiber and fat content of the flour. The result of the mineral composition of the processed potato reveals that there is a marked reduction of sodium and potassium level in the flour obtained by boiling prior to drying compared to flourobtained by drying the raw potato tubers. The antioxidant activity of the flour on the other hand reveals that the drying methods (oven or sun drying) do not bring a remarkable change in the antioxidant activities of the flour. However, the decline in the antioxidant activity is only noticeable in the flour obtained after boiling compared with the flour obtained from tubers in its raw agricultural state. Better nutritional value and antioxidant potential of sweet potato flour could be derived when tubers are dried in its raw agricultural state without boiling.


Publisher Hindawi Publishing Corporation
Publication Type Journal
Publication Title Inhibitory Effect Of Aqueous Extract Of Stem Bark Of Cissus Populnea On Ferrous Sulphate- And Sodium Nitroprusside-Induced Oxidative Stress In Rat’s
Publication Authors Akomolafe S.F, Oboh G, Akindahunsi A.A, Akinyemi A.J. and Tade O. G.
Year Published 2013
Abstract

Cissus populnea are plants associated with a myriad of medicinal uses in different parts of the world and are good sources of carotenoids, triterpenoids, and ascorbicacid. isstudy seeksto investigatetheinhibitory effect of waterextractible phytochemicals fromstembarkof C. populnea on FeSO4-andsodiumnitroprusside-(SNP-) induced lipid peroxidation in rat testis invitro. Aqueous extract of the plant was prepared (1:100w/v) and used for subsequent analysis. The antioxidant properties and inhibitory effect of the extractonFeSO4 -andSNP-induced lipid peroxidation in rat testes were determined invitro. The results revealed that the extract was able to scavenge DPPH radical and chelate Fe2+ and also had a high reducing power. Furthermore, the incubation of the testes tissue homogenate in the presence of FeSO4 and SNP, respectively, caused a signiĕcant increase in the malondialdehyde (MDA) contents of the testes. However, the aqueous extract of the stem bark of C. populnea caused a signiĕcant decrease in the MDA contents of both Fe2+ (EC50=0.027mg/mL) and SNP- (EC50 =0.22mg/mL) induced lipid peroxidation in the rat testes homogenates in a dose-dependent manner. e water extractible phytochemicals from C. populnea protect the testes from oxidative stress, and this could be attributed to their high antioxidant activity: DPPH-scavenging ability and Fe2+ -chelating and -reducing power. Therefore, oxidatively stress testes could be potentially managed/ prevented by this plant


Publisher ETOX & IEPT, SASc.
Publication Type Journal
Publication Title Testicular Membrane Lipid Damage By Complex Mixture Of Leachate From Municipal Battery Recycling Site As Indication Of Idiopathic Male Infertility In Rat.
Publication Authors Akintunde J.K. Oboh G. and Akindahunsi A.A.
Year Published 2013
Abstract

Leachate from a municipal battery recycling site is a potent source of mixed-metal released into the environment. The present study investigated the degree at which mixed-metal exposure to the municipal auto-battery leachate (MABL) and to the Elewi Odo municipal auto-battery recycling site leachate (EOMABRL) affected the lipid membrane of the testes in in vitro experiment. The results showed elevated level of mixed-metals over the permissible levels in drinking water, as recommended by regulatory authorities. In the leachate samples, the levels of malondialdehyde (MDA), a biomarker of lipid damage, was significantly (p<0.05) increased in rat testes in a dose-dependent manner. MDA induced by the municipal auto-battery leachate (MABL) was significantly (p<0.05) higher than the leachate from Elewi Odo municipal auto-battery recycling site (EOMABRL). The testicular lipid membrane capacity was compromised following treatment with leachate from the municipal battery recycling site, implicating mixed-metal exposure as the causative agent of testicular damage and male infertility.


Publisher Nil
Publication Type Journal
Publication Title Effect Of Cooking On The Antioxidant Properties Of Two Varieties Of Bitter Yam (Dioscorea Dumetorum).
Publication Authors Adedayo B.C., Oboh G. and Akindahunsi A.A.
Year Published 2013
Abstract

Nil


Publisher Nil
Publication Type Journal
Publication Title HPLC/DAD/MS Phenolic Profile, Antioxidant Activities And Inhibitory Action Of Struchium Sparganophora (Linn) And Telfairia Occidentalis (Hook. F) Against Low Density Lipoprotein Oxidation.
Publication Authors Salawu, S.O., Sanni, D.M., Akindahunsi, A.A
Year Published 2013
Abstract

The present study sought to investigate the phenolic composition of Struchium sparganophora and Telfairia occidentalis extracts using HPLC/DAD/MS techniques, evaluate their radical scavenging activities and inhibitory action against copper induced human Low Density Lipoprotein (LDL) oxidation. The chromatographic analysis revealed the presence of apigenin derivatives, chlorogenic acid and three dicaffeoyl derivatives in Strcuchium sparganophora while chlorogenic acid, rutin and three Kaempferol derivatives were identified Telfairia occidentals. ABTS radical scavenging activities and DPPH radical scavenging activities are higher in Telfairia occidentalis compared with Struchium sparjanophora. Also, the total phenolic content was higher in Telfairia occidentalis compared with Strcuchium sparganophora. Similarly, the inhibitory action of the vegetable extracts against copper induced human LDL oxidation showed that Telfairia occidentalis posses a higher inhibitory action (63%) compared with Struchium sparjanophora (55%). Therefore, the antioxidant activities and the ability of the extract to inhibit the copper induced human LDL oxidation shows that the studied vegetables could be harnessed in the management of some coronary and cardiovascular disorders.


Publisher Interational Scholars Journals
Publication Type Journal
Publication Title Antioxidant Activities And Inhibitory Action Of Solanum Macrocarpon And Hibiscus Esculentus Phenolic Containing Leaf Extracts Against Lipid Oxidation.
Publication Authors Salawu, S.O., Akindahunsi, AA..,. Ibukun, E.O and Duodu K.G
Year Published 2013
Abstract

There is a growing interest in preventive health care for the development and evaluation of natural antioxidants from plant food. Therefore, this study sought to investigate the phenolic composition of Solanum macrocarpon and Hibiscus esculentus leaves using HPLC/DAD/MS techniques, evaluate the radical scavenging activities, ferric reducing properties and assess the inhibitory action of the vegetable extracts against Low Density Lipoprotein (LDL) and egg yolk lipid- rich oxidation. The HPLC analysis revealed the presence of cynamoyl derivative, two chlorogenic acid derivatatives, rutin and Kaempferol-3- rutinoside in S. macrocarpon and five dicaffeoyl derivatives, unidentified flavonoid and isoquercitrin in Hibiscus esculentus leaf. The result indicated that the total phenolic content (mg tannic acid equivalent/g) and 2, 2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging (μmol trolox equivalent/g) activities were higher in Hibiscus esculentus phenolic extract (12.96± 0.04; 2.44E-05 ± 4.52E-06), respectively. Similarly, ferric reducing antioxidant power (mg ascorbic acid/g ) and 1, 1-diphenyly-2- picrylhydrazyl radical scavenging (μmol trolox equivalent/g ) activities were slightly higher in Hibiscus esculentus extract (10.60 ± 0.14; 2.68E-05 ± 1.56E-05), respectively. The result also showed that the studied vegetable extracts inhibited both human LDL and lipid rich egg homogenate oxidation. Overall, the antioxidant action and protection against lipid oxidation could be linked with the presence of the phenolic compounds in the vegetable extracts and the consumption will be of immense benefit in the prevention of a number of free radical mediated diseases.


Publisher Nil
Publication Type Journal
Publication Title Effect Of Methanolic Leaf Extract Of Parkia Biglobosa On Some Biochemical Indices And Hemodynamic Parameters In Rats,
Publication Authors Olaleye MT., Komolafe K., Akindahunsi AA.
Year Published 2013
Abstract

To provide justification for the use of plant in ethnomedicine, the leaf extract of P. biglobosa was investigated for its effects on some biochemical indices, antioxidant status and hemodynamic parameters in rats. 80% methanolic extract of P. biglobosa leaf (PBE, 75mg/kg body weight) was administered once daily to experimental animals over a 30-day period. The effect on biochemical parameters like aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum alkaline phosphatase (ALP), albumin, total-, direct bilirubin, urea, creatinine and lipid profile was assessed. Hepatic activity of glutathione peroxidase (GPx) and levels of lipid peroxidation (LPO) and reduced glutathione (GSH) were quantified to gain insight into the antioxidant status. Effect on hemodynamic parameters was investigated in other groups of ratspretreated with PBE (50- or 75 mg/kg) or ramipril (10 mg/kg) for fifteen consecutive days. PBE reduced serum ALT and AST levels after 10 days of treatment but with no change in hepatic activities of enzymes. Significant reduction (P<0.01) in serum levels of direct- and total bilirubin, cholesterol, triglyceride, LDL-cholesterol was also observed after 15 days of treatment in rats while other indices were not significantly affected. Improved antioxidant status was shown by the significantly reduced LPO and increased GPx activity. Futhermore, PBE demonstrated hypotensive effect by reducing the mean arterial blood pressure and heart rate in rats. The results of this study strongly indicate the hypotensive effect an

d the safety of PBE to vital organs at the dosages evaluated and there by scientifically support its traditional use. 


Publisher Nil
Publication Type Journal
Publication Title Rutin And Quercetin Show Greater Efficacy Than Nifedipin In Regulating Hemodynamic Indices And Ameliorating Redox And Metabolite Imbalances In Salt-induced Hypertensive Rats.
Publication Authors Olaleye, M.T., Crown, O.O., Akinmoladun, A.C. and Akindahunsi, A.A.
Year Published 2013
Abstract

Nil


Publisher Nil
Publication Type Journal
Publication Title Lipid-lowering Effect Of Parkia Biglobosa Leaf Saponins In Triton-X 1339-induced Hyperlipidemic Rats.
Publication Authors Komolafe K, Olaleye MT., Fasan TI., Elekofehinti OO., Saliu J. A., Akindahunsi A. A.
Year Published 2013
Abstract

Parkia biglobosa has popular ethnomedicinal use in the treatment of cardiovascular-related disorders. Hyperlipidemia has been described as the greatest risk factor contributing to the prevalence and severity of coronary heart diseases. Here, we investigated the effect of saponins from Parkia biglobosa leaf on triton-induced hyperlipidemic rats. Hyperlipidemia was induced in wistar rats by single intraperitoneal (i.p) injections of Triton X-1339 at a dose of 350 mg/kg b.w following oral pretreatment with P. biglobosasaponin mixture (PBS) (30-or 60mg/kg/day) or nicotinic acid (500 mg/kg/day) for five days. Another group of rats received PBS (40 mg/kg/day) alone. Animals were euthanized by decapitation 20 h after triton induction to harvest serum used in biochemical analysis. PBS prevented triton- induced increase in serum cholesterol, triglyceride, low density lipoprotein (LDL) and decrease in high density lipoprotein (HDL) in a statistically significant manner (P<0.05) and reversed the elevated serum LDL/HDL ratio comparably to nicotinic acid. PBS alone also caused significantly reduced serum lipid profile in rats. The results demonstrated the mitigation of triton-induced hyperlipidemia in rats by PBS. Consequently, we can hypothesize that part of the P. biglobosa-mediated therapeutic effectsis associated with its hypolipidemic components.


Publisher Nil
Publication Type Journal
Publication Title Evaluation Of The Toxicological Profile Of The Leaves And Young Twigs Of Caesalpinia Bonduc (Linn) Roxb.
Publication Authors Olubanke O Ogunlana, Oluseyi E Ogunlana, Adejuwon A Adeneye, OAC Udo-Chijioke, TI Dare-Olipede, Joseph A Olagunju, and Afolabi A Akindahunsi
Year Published 2013
Abstract
Acute and sub-acute toxicological effects of ethanolic extract of the leaves and young twigs of Caesalpinia bonduc were carried out on albino rats. Single extract doses from 2000 to 5000 mg/kg body weight were administered orally and monitored for 14 days in acute study, while extract doses from 200 to 1600 mg/kg body weight were orally administered daily for 28 days in sub-acute study and recovery was assessed 14 days after dosing. Biochemical, haematological and histopathological examinations were carried out. There was no mortality in the experimental animals in all acute treatment doses. However, there were significant alterations in the biomarkers and induced cellular damage to the liver in all acute treatment doses. In the sub-acute toxicity treatment, the assessed biomarkers were unaffected at extract dose of 200 mg/kg body weight compared to control, while significant changes were observed in rats administered with extract doses of 400 mg/kg body weight and above. No significant difference was observed between the tested groups and the recovery groups in the sub-acute toxicity study. In conclusion, the ethanolic extract of C. bonduc could be toxic to selected organs of the rat body in acute and sub-acute treatments.

Publisher Nil
Publication Type Journal
Publication Title Phenolic Composition And Inhibitory Activity Of Mangifera Indica And Mucuna Urens Seeds Extracts Against Key Enzymes Linked To The Pathology And Complications Of Type 2 Diabetes.
Publication Authors Irondi E.A., Oboh G., Akindahunsi A.A. Boligon A.A. and Athayde M.L.
Year Published 2014
Abstract
Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase>α-glucosidase>α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes.

Publisher Hindawi Publishing Corporation
Publication Type Journal
Publication Title Improvement Of Mitochondrial Stress And Neuroprotectivity Of Parkia Biglobosa Leaf In Rats’ Brain Hippocampal Slices
Publication Authors Komolafe K, Olaleye MT, Seeger RL, Carvalho FB, Boligon AA, Athayde ML, Akindahunsi AA, Rocha JBT
Year Published 2014
Abstract
Objective. Methanolic leaf extracts of Parkia biglobosa, PBE, and one of its major polyphenolic constituents, catechin, were investigated for their protective effects against neurotoxicity induced by different agents on rat brain hippocampal slices and isolated mitochondria. Methods. Hippocampal slices were preincubated with PBE (25, 50, 100, or 200 µg/mL) or catechin (1, 5, or 10 µg/mL) for 30 min followed by further incubation with 300 µM H2O2, 300 µM SNP, or 200 µM PbCl2 for 1 h. Effects of PBE and catechin on SNP- or CaCl2-induced brain mitochondrial ROS formation and mitochondrial membrane potential (ΔΨm) were also determined. Results. PBE and catechin decreased basal ROS generation in slices and blunted the prooxidant effects of neurotoxicants on membrane lipid peroxidation and nonprotein thiol contents. PBE rescued hippocampal cellular viability from SNP damage and caused a significant boost in hippocampus Na+, K+-ATPase activity but with no effect on the acetylcholinesterase activity. Both PBE and catechin also mitigated SNP- or CaCl2-dependent mitochondrial ROS generation. Measurement by safranine fluorescence however showed that the mild depolarization of the ΔΨm by PBE was independent of catechin. Conclusion. The results suggest that the neuroprotective effect of PBE is dependent on its constituent antioxidants and mild mitochondrial depolarization propensity.

Publisher Elsevier
Publication Type Journal
Publication Title In Vitro Antioxidant Activity And Effect Of Parkia Biglobosa Bark Extract On Mitochondrial Redox Status.
Publication Authors Komolafe K, Olaleye MT, Boligon AA, Athayde ML, Akindahunsi AA, Rocha JBT.
Year Published 2014
Abstract

Aqueous-methanolic extract of Parkia biglobosa bark (PBB) was screened for its polyphenolic constituents, in vitro antioxidant activity, and effect on mitochondria redox status. The in vitro antioxidant activity was assessed by using the scavenging abilities and the reducing powers of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2 0 -azino-bis (3 ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt radical cation against Fe3þ. Subsequently, the ability of PBB to inhibit lipid peroxidation induced by FeSO4 (10 mm) and its metal-chelating potential were investigated. The effects of the extract on basal reactive oxygen species (ROS) generation and on the mitochondrial membrane potential (DJm) in isolated mitochondria were determined by using 20,7 0 -dichlorodihy- drofluorescin (DCFH) oxidation and safranin fluorescence, respectively. PBB mitigated the Fe(II)-induced lipid peroxidation in rat tissues and showed dose-dependent scavenging of DPPH (IC 50: 98.3310.0 mg/mL) and ABTS. (trolox equivalent antioxidant)


Publisher Elsevier
Publication Type Journal
Publication Title Phenolic Composition And Inhibitory Activity Of Mangifera Indica And Mucuna Urens Seeds Extracts Against Key Enzymes Linked To The Pathology And Complications Of Type 2 Diabetes.
Publication Authors Irondi E.A., Oboh G., Akindahunsi A.A. Boligon A.A. and Athayde M.L.
Year Published 2014
Abstract
Objective: To investigate the phenolic compounds composition and the inhibitory activity of Mangifera indica (M. indica) and Mucuna urens (M. urens) seeds extracts against some key enzymes (α-amylase, α-glucosidase and aldose reductase) implicated in the pathology and complications of type 2 diabetes in vitro. Methods: Reverse phase chromatographic quantification of the major flavonoids and phenolic acids in the seeds extracts was carried out using high performance liquid chromatography coupled with diode array detection. The inhibitory activities of the seeds extracts against α-amylase and α-glucosidase were estimated using soluble starch and ρ-nitrophenylglucopyranoside as their respective substrates. Inhibition of aldose reductase activity by the extracts was assayed using partially purified lens homogenate of normal male rat as source of enzyme; inhibition of Fe2+-induced lipid peroxidation by extracts was tested in rat pancreas homogenate. Results: The chromatography result revealed that extracts of both seeds had appreciable levels of some major flavonoids and phenolic acids of pharmacological importance, including gallic acid, chlorogenic acid, caffeic acid, ellagic acid, catechin, rutin, quercitrin, quercetin and kaempferol. Extracts of both seeds effectively inhibited α-amylase, α-glucosidase and aldose reductase activities in a dose-dependent manner, having inhibitory preference for these enzymes in the order of aldose reductase>α-glucosidase>α-amylase. With lower half-maximal inhibitory concentrations (IC50) against α-amylase, α-glucosidase, and aldose reductase, M. indica had stronger inhibitory potency against these enzymes than M. urens. Extracts of both seeds also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, with M. indica being more potent than M. urens. Conclusions: The results obtained provide support for a possible use of M. indica and M. urens seeds in managing hyperglycemia and preventing the complications associated with it in type 2 diabetes. 

Publisher Elsevier
Publication Type Journal
Publication Title Possible Involvement Of Membrane Lipids Peroxidation And Oxidation Of Catalytically Essential Thiols Of The Cerebral Transmembrane Sodium Pump As Component Mechanisms Of Iron-mediated Oxidative Stress-linked Dysfunction Of The Pump's Activity.
Publication Authors Omotayo TI, Akinyemi GS, Omololu PA, Ajayi BO, Akindahunsi AA, Rocha JB, Kade IJ
Year Published 2015
Abstract

The precise molecular events defining the complex role of oxidative stress in the inactivation of the cerebral sodium pump in radical-induced neurodegenerative diseases is yet to be fully clarified and thus still open. Herein we investigated the modulation of the activity of the cerebral transmembrane electrogenic enzyme in Fe(2+)-mediated in vitro oxidative stress model. The results show that Fe(2+) inhibited the transmembrane enzyme in a concentration dependent manner and this effect was accompanied by a biphasic generation of aldehydic product of lipid peroxidation. While dithiothreitol prevented both Fe(2+) inhibitory effect on the pump and lipid peroxidation, vitamin E prevented only lipid peroxidation but not inhibition of the pump. Besides, malondialdehyde (MDA) inhibited the pump by a mechanism not related to oxidation of its critical thiols. Apparently, the low activity of the pump in degenerative diseases mediated by Fe(2+) may involve complex multi-component mechanisms which may partly involve an initial oxidation of the critical thiols of the enzyme directly mediated by Fe(2+) and during severe progression of such diseases; aldehydic products of lipid peroxidation such as MDA may further exacerbate this inhibitory effect by a mechanism that is likely not related to the oxidation of the catalytically essential thiols of the ouabain-sensitive cerebral electrogenic pump.


Publisher Elsevier
Publication Type Journal
Publication Title Ethylacetate Extract Of Red Onion (Allium Cepa L.) Tunic Affects Hemodynamic Parameters In Rats. Food Science And Human Wellness
Publication Authors Olayeriju OS, Olaleye MT, Crown OO, Komolafe K, Boligon AA, Athayde ML, Akindahunsi AA.
Year Published 2015
Abstract
The effects of ethylacetate extract of red onion (Allium cepa) tunic (ACTE) on some hemodynamic and biochemical parameters were evaluated in normotensive albino rats. Blood pressure parameters were determined in anaesthetized rats orally administered ACTE (10-, 20-, or 40 mg/kg) or ramipril (1 mg/kg) once daily for 14 days. Respectively, 10-, 20-, or 40 mg/kg ACTE produced significant (P < 0.01), dose-dependent fall in systolic blood pressure, SBP (21%, 27%, 33%), diastolic blood pressure, DBP (6%, 10%, 16%), pulse pressure, PP (42%, 49%, 56%), mean arterial blood pressure, MAPB (13%, 18%, 23%) and heart rate, HR (4%, 5%, 7%). The highest effective dose (40 mg/kg) compared well with ramipril (1 mg/kg) with regards to SBP (41%), DBP (19%), PP (70%), MABP (29%) and HR (10%). Similar trends (decreases) were recorded for 40 mg/kg ACTE and ramipril, respectively, as regards the activities of serum enzymes: creatine kinase (60% and 65%), ALT (18% and 14%) and ALP (28% and 16%). HPLC fingerprints of the flavonoid-rich ACTE revealed that flavonols: quercetin, quercitrin, isoquercitrin, rutin and kaempferol are the active flavonoids. The results demonstrate the hypotensive effect of A. cepa tunic flavonoids initiating further investigation of their individual or synergistic contribution(s) to the observed effects.

Publisher Nil
Publication Type Journal
Publication Title Nutraceutical Potential And Sensory Acceptability Of Unripe Plantain-Millet Composite Flour Blends. Food Science And Quality Management
Publication Authors Salawu, S.O, Shaibu, R, Akindahunsi, A. A, Boligon, A.A, Athayde, M.L
Year Published 2015
Abstract

There is an increasing need globally to eat foods that contain arrays of health promoting phytochemicals as alternatives to synthetic drugs. Therefore, the present study sought to assess the nutritional and antioxidant potentials of unripe plantain (UP) and millet (M) composite blends (UP: M); 100:0, 80:20, 60:40, 40:60, 20:80 and 0:100. Some of the evaluated proximate indices (fat, protein, and fiber) gave high values as the composition of millet increases in the blends. On the contrary, the result showed an increasing value in the level of some minerals (sodium, calcium, potassium and magnesium) as the inclusion of unripe plantain increases in the blend, with the exception of phosphorous. Physico-chemicalproperties of the blends did not show any particular trend with the proportion of either plantain or millet inthe blend. The result of the sensory properties of the porridge prepared from the blend showed a varied rating, with better overall acceptability for two blends (40UP:60M, 20UP:80M). HPLC/DAD analyses of millet and unripe plantain revealed the presence of some flavonoids and phenolic acids. Antioxidant indices were also high as the percentage inclusion of millet increases in the blend. Results from this investigation revealed that millet contributes higher antioxidant potential and is a better source of protein and fat, while unripe plantain have higher amount of minerals. This by implication is that the combination of these plant food most especially two of the blends (40UP:60M and 20UP:80M) would serve well as a functional food which could be harnessed as inthe management of free radical mediated diseases. 


Publisher Nil
Publication Type Journal
Publication Title Antioxidant Potential, Phenolic Profile And Nutrient Composition Of Flesh And Peels From Nigerian White And Purple Skinned Sweet Potato (Ipomea Batatas L.).
Publication Authors Salawu, S.O, Udi, E, Akindahunsi, A.A, Boligon, AA. and Margareth L. Athayde.
Year Published 2015
Abstract

There is an increasing need globally to eat foods that contain arrays of health promoting phytochemicals as alternatives to synthetic drugs. Therefore, the present study sought to assess the nutritional and antioxidant potentials of unripe plantain (UP) and millet (M) composite blends (UP: M); 100:0, 80:20, 60:40, 40:60, 20:80 and 0:100. Some of the evaluated proximate indices (fat, protein, and fiber) gave high values as the composition of millet increases in the blends. On the contrary, the result showed an increasing value in the levelof some minerals (sodium, calcium, potassium and magnesium) as the inclusion of unripe plantain increases in the blend, with the exception of phosphorous. Physico-chemical properties of the blends did not show any particular trend with the proportion of either plantain or millet in the blend. The result of the sensory properties ofthe porridge prepared from the blend showed a varied rating, with better overall acceptability for two blends (40UP:60M, 20UP:80M). HPLC/DAD analyses of millet and unripe plantain revealed the presence of some flavonoids and phenolic acids. Antioxidant indices were also high as the percentage inclusion of millet increases in the blend. Results from this investigation revealed that millet contributes higher antioxidant potential and is a better source of protein and fat, while unripe plantain have higher amount of minerals. This by implication is that the combination of these plant food most especially two of the blends (40UP:60M and 20UP:80M) would serve well as a functional food which could be harnessed as in the management of free radical mediated diseases. 


Publisher Springer
Publication Type Journal
Publication Title Inhibition Of Key Markers Linked With Spermatogenesis And Cellular ATP By Subchronic Exposure To Leachate In A Rat Model.
Publication Authors Akintunde J. K., Oboh G and Akindahunsi A.A.
Year Published 2015
Abstract
Few or no studies have measured the toxic effects of subchronic exposure to leachate using key markers linked with spermatogenesis and cellular adenosine triphosphate (ATP) in an experimental rat model. This study was undertaken to evaluate the toxic effects of leachate obtained from the Elewi Odo municipal battery-recycling site (EOMABRL) on male reproductive function using testicular hormones and biomarker of cellular ATP. EOMABRL was administered at 0, 20, 40, 60, 80, and 100 % concentrations to adult male rats for 60 days. After exposure, serum was collected for hormonal biochemistry assays, and testes were collected to determine the activity of xanthine oxidase (XO) and lactate dehydrogenase (LDH). Exposure of animals to EOMABRL resulted in a 519.7, 285.7, 569.1, 606.1, and 1,793.2 % increase in XO activity with a sequential decrease in LDH activity (marker of cellular ATP) by 44.1, 55.9, 61.4, 69.3, and 89.7 %, respectively, compared with the control. Furthermore, EOMABRL caused a significant inhibitory effect on serum luteinizing hormone, follicle-stimulating hormone, and testosterone levels. We conclude that some possible mechanisms by which EOMABRL elicits toxicity in male rat testes could be through inhibition of LDH activity and depletion of serum hormone levels.

Publisher Nil
Publication Type Journal
Publication Title Phenolics Composition And Antidiabetic Property Of Brachystegia Eurycoma Seed Flour In High-Fat Low-Dose Streptozotocin-Induced Type 2 Diabetes In Rats”
Publication Authors Irondi E.A., Oboh G., Akindahunsi A.A. Boligon A.A. and Athayde M.L
Year Published 2015
Abstract

Objective: To quantify some major pharmacologically important flavonoids and phenolic acids in Brachystegia eurycoma seed flour (BESF) and evaluate its antidiabetic activity in type 2 diabetic rats.  Method: Flavonoids and phenolic acids were quantified using a reverse-phase high pressure liquid chromatrography coupled with diode array detection. Type 2 diabetes was induced in  rats using high-fat diet, low-dose streptozotocin (HFD/STZ) model, by feeding the rats with  HFD for 2 weeks followed by single dose administration of STZ (40 mg/kg body weight,  intraperitoneally). The diabetic rats were later fed BESF-supplemented (10% and 20%) diets, or administered with metformin (25 mg/kg b.w.) for 21 days; the control rats were fed basal diet during this period. After the dietary regimen, the rats were sacrificed, and their blood, liver and pancreas samples were collected for biochemical assays. Results:  The flavonoids (catechin, rutin, quercitrin, quercetin and kaempferol) and phenolic acids (gallic acid, caffeic, chlorogenic and ellagic acid) were abundant in BESF. BESF- supplemented diets (BESF-SD) significantly (P< 0.05) decreased the levels of fasting blood glucose (FBG); ameliorated the lipid profile, and significantly (P< 0.05) increased the hepatic glycogen of the diabetic rats. BESF-SD also significantly (P < 0.05) attenuated the oxidative stress induced by STZ in the liver and pancreas of the diabetic rats. The ameliorative effects of 20% BESF-SD compared (P> 0.05) with metformin administration in some of the biomarkers. Conclusion: The flavonoids and phenolic acids in BESF may have acted synergistically to produce the observed antidiabetic effects. BESF could therefore be an effective and affordable dietary therapy for the management of T2DM; and an excellent source for drug discovery. 


Publisher CrossMark
Publication Type Journal
Publication Title Tetracarpidium Conophorum Ameliorates Oxidative Reproductive Toxicity Induced By Ethanol In Male Rats.
Publication Authors Akomolafe, S F; Oboh, G ; Akindahunsi, A A, and Afolayan, A J
Year Published 2015
Abstract

Background; Tetracarpidium conophorum (Mull. Arg.) Hutch. & Dalz is one of the many medicinal plants used for ages in folklore as male fertility enhancers. The current study evaluates the effect of the plant leaf extract on alcohol - induced reproductive toxicity in male rats.  Methods; Thirty rats were randomly divided into six groups of five animals each; Group 1 (positive control) received normal saline only; Group 2 (ethanol alone) were given only 30 % ethanol orally at 7 ml/kg body weight per day, thrice in a week; Group 3, 4, 5 were given ethanol and co-treated with 50 mg/kg, 500 mg/kg and 1000 mg/kg body weight of leaf extract respectively while Group 6 were given ethanol and co-treated with a fertility drug, clomiphene citrate. All the drugs were given daily and the experiment lasted for twenty one consecutive days. Results; Alcohol ingestion resulted in a significant (p  < 0.05) decrease in water, food intake and marked elevation of lipid peroxidation as assessed by the accumulation of malondialdehyde (MDA) in the reproductive tissues. Precisely, MDA level was elevated in the testis, epididymis, seminal vesicle and prostate gland by 81 %, 63 %, 95 % and 91 %, respectively. Furthermore, levels of total protein, reduced glutathione (GSH), vitamin C and activities of antioxidant enzymes in the reproductive tissues were significantly (p < 0.0001) reduced in ethanol-ingested rats. Interestingly, co-administration of T. conophorum with ethanol led to almost complete inhibition of lipid peroxidation thereby enhancing antioxidant status of the reproductive tissues. Conclusion; Overall, T. conophorum ameliorates oxidative reproductive toxicity induced by ethanol in male rats and its ameliorative effect comparable well with the fertility drug, clomiphene citrate.


Publisher Elsevier
Publication Type Journal
Publication Title Sub-chronic Exposure To EOMABRS Leachate Induces Germinal Epithelial Cell Lesions, Sperm Abnormalities And Oxidative Damage In Rats
Publication Authors Akintunde J. K., Oboh G and Akindahunsi A.A.
Year Published 2015
Abstract

Objectives; To explore the possible link between reproductive abnormalities among men and exposure of toxic chemicals in the environment. Methods; The study investigated the sperm functions and the antioxidant defence system of rats exposed to leachate obtained from Elewi Odo municipal battery recycling site (EOMABRSL) via oral route. Results; EOMABRSL had significant effects on both absolute and relative testicular weight. Formation of sperm abnormalities was observed following EOMABRSL exposure. Antioxidant enzymes including superoxide dismutase and catalase were significantly altered in the testes resulting into increased lipid peroxidation. Reduced glutathione (GSH) levels were found to be significantly (P < 0.05) depleted relative to the control group. Considerable necrosis of leydig cells and loss of germ cells in the seminiferous tubules with the clumping of interstitial space were seen in EOMABRSL-treated rats. The mechanism of toxicity is linked to individual, synergistic, antagonistic, competitive or collective interaction of the metals with normal testicular biochemical processes.

Conclusion; The study concluded that possible mechanisms by which EOMABRSL at the investigated doses elicits spermatotoxicity could be linked to the testicular oxidative stress and damage to germinal epithelial cells by mixed-metal exposure. However, this may suggest possible reproductive health hazards in subjects with environmental or industrial exposure.


Publisher Elsevier
Publication Type Journal
Publication Title Methanol Extracts Of Brachystegia Eurycoma And Detarium Microcarpum Seeds Flours Inhibit Some Key Enzymes Linked To The Pathology And Complications Of Type 2 Diabetes In Vitro
Publication Authors Irondi E.A., Oboh G., Akindahunsi A.A.
Year Published 2015
Abstract

The inhibitory effect of methanol extracts of Brachystegia eurycoma and Detarium microcarpum seeds flours on some key enzymes [ -amylase, -glucosidase and aldose reductase (AR)linked to the pathology and complications of type 2 diabetes (T2D); and their antioxidant properties were evaluated. The antioxidant properties evaluated were DPPH • and ABTS • + scavenging abilities, reducing power, and antioxidant phytochemicals (total phenolics, tannins, total flavonoids and total saponins). Extracts of both flours inhibited -amylase, -glucosidase and AR in a dose-dependent manner. The half-maximal inhibitory concentrations (IC 50) of B. eurycoma on -amylase, -glucosidase, AR and lipid peroxidation were lower than those of D. microcarpum, indicating that it had stronger inhibitory potency than D. microcarpum. B. eurycoma also had significantly (P < 0.05) higher DPPH • and ABTS • + scavenging abilities, and reducing power than D. microcarpum. The antioxidant phytochemicals (totalphenolics, tannins, total flavonoids and total saponins) were also significantly ( P < 0.05) higher in B. eurycoma than D. microcarpum . The inhibitory effect of B. eurycoma and D. microcarpum extracts on -amylase, -glucosidase and ARactivitiesmay be attributed to the combined action of their polyphenols and total saponins, and this may be a possible mechanism of action providing supportfor their use in managing hyperglycemia and the complications of T2D.


Publisher Nil
Publication Type Journal
Publication Title Contribution Of Musa Paradisiaca In The Inhibition Of α-amylase, α-glucosidase And Angiotensin-I Converting Enzyme In Streptozotocin Induced Rats.
Publication Authors Shodehinde SA, Ademiluyi AO, Oboh G, Akindahunsi AA
Year Published 2015
Abstract

Nil


Publisher Nil
Publication Type Journal
Publication Title Effect Of Dietary Supplementation Of Ginger And Turmeric Rhizomes On Ectonucleotidases, Adenosine Deaminase And Acetylcholinesterase Activities In Synaptosomes From The Cerebral Cortex Of Hypertensive Rats
Publication Authors Akinyemi A. J., Thome G. R., Morsch V. M., Stefanello N., Costa P., Cardoso A., Goularte J. F., Belló-Klein A., Akindahunsi A.A. Oboh G and Schetinger R.M.C.
Year Published 2015
Abstract

Nil


Publisher Nil
Publication Type Journal
Publication Title Estimated Glycemic Indices And Inhibitory Action Of Some Yam (Dioscorea Spp.) Products On Key Enzymes Linked With Type–2 Diabetes
Publication Authors Adedayo B.C., Oboh G. and Akindahunsi A.A
Year Published 2015
Abstract

Nil


Publisher Hindawi Publishing Corporation
Publication Type Journal
Publication Title Antiperoxidative Activity Of Tetracarpidium Conophorum Leaf Extract In Reproductive Organs Of Male Rats.
Publication Authors Akomolafe S.F., Oboh G., Akindahunsi A.A. and Afolayan A.J.
Year Published 2015
Abstract

Tetracarpidium conophorum (Mull. Arg.) Hutch. & Dalz is one of the many medicinal plants used in folklore as male fertility enhancers. This research was aimed at evaluating the anti-peroxidative activity of the leaves of this plant by determining their capacity to reduce malondialdehyde (MDA) level in reproductive organs and accessory glands of rats. Adult male rats were administered orally with the aqueous leaf extract from T. conophorum at 50, 500 and 1000 mg/kg body weight for 21 consecutive days while clomiphene citrate (1.04 mg/kg body weight), a fertility drug was used as standard. The results of the study indicated that there was increase in relative organ weight, body weight, mean total food and water consumed by the treated groups. Testicular MDA level was highly significantly different from that of the control () although a tentatively decreased MDA level was observed. However, MDA levels in the reproductive accessory glands, epididymis, seminal vesicle and prostate gland were insignificantly () lower than those of controls. The highest percentage decrease of MDA level (66.35, 42.68, 62.50 and 63.36%) was observed at the highest concentration of the extract (1000 mg/kg) in the testis, epididymis, seminal vesicle and prostate gland respectively. These values were two-fold greater than the values obtained for the standard drug. Interestingly, the treatment of rats with the extract significantly increased the activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and the levels of GSH, vitamin C and total protein. Collectively, the results suggest that the extract from T. conophorum leaves had greater capacity to reduce lipid peroxidation in reproductive organs and accessory glands and thus, this plant may be useful in the treatment/management of reproductive cellular damage involving reactive oxygen species.


Publisher Hindawi Publishing Corporation
Publication Type Journal
Publication Title Antiperoxidative Activity Of Tetracarpidium Conophorum Leaf Extract In Reproductive Organs Of Male Rats.
Publication Authors Akomolafe S.F., Oboh G., Akindahunsi A.A. and Afolayan A.J.
Year Published 2015
Abstract

Tetracarpidium conophorum (Mull. Arg.) Hutch. & Dalz is one of the many medicinal plants used in folklore as male fertility enhancers. This research was aimed at evaluating the anti-peroxidative activity of the leaves of this plant by determining their capacity to reduce malondialdehyde (MDA) level in reproductive organs and accessory glands of rats. Adult male rats were administered orally with the aqueous leaf extract from T. conophorum at 50, 500 and 1000 mg/kg body weight for 21 consecutive days while clomiphene citrate (1.04 mg/kg body weight), a fertility drug was used as standard. The results of the study indicated that there was increase in relative organ weight, body weight, mean total food and water consumed by the treated groups. Testicular MDA level was highly significantly different from that of the control () although a tentatively decreased MDA level was observed. However, MDA levels in the reproductive accessory glands, epididymis, seminal vesicle and prostate gland were insignificantly () lower than those of controls. The highest percentage decrease of MDA level (66.35, 42.68, 62.50 and 63.36%) was observed at the highest concentration of the extract (1000 mg/kg) in the testis, epididymis, seminal vesicle and prostate gland respectively. These values were two-fold greater than the values obtained for the standard drug. Interestingly, the treatment of rats with the extract significantly increased the activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and the levels of GSH, vitamin C and total protein. Collectively, the results suggest that the extract from T. conophorum leaves had greater capacity to reduce lipid peroxidation in reproductive organs and accessory glands and thus, this plant may be useful in the treatment/management of reproductive cellular damage involving reactive oxygen species.


Publisher Nil
Publication Type Journal
Publication Title Tetracarpidium Conophorum Ameliorates Oxidative Reproductive Toxicity Induced By Ethanol In Male Rats.
Publication Authors Akomolafe SF, Oboh G, Akindahunsi AA, Afolayan AJ
Year Published 2015
Abstract
Background; Tetracarpidium conophorum (Mull. Arg.) Hutch. & Dalz is one of the many medicinal plants used for ages in folklore as male fertility enhancers. The current study evaluates the effect of the plant leaf extract on alcohol - induced reproductive toxicity in male rats. Methods; Thirty rats were randomly divided into six groups of five animals each; Group 1 (positive control) received normal saline only; Group 2 (ethanol alone) were given only 30 % ethanol orally at 7 ml/kg body weight per day, thrice in a week; Group 3, 4, 5 were given ethanol and co-treated with 50 mg/kg, 500 mg/kg and 1000 mg/kg body weight of leaf extract respectively while Group 6 were given ethanol and co-treated with a fertility drug, clomiphene citrate. All the drugs were given daily and the experiment lasted for twenty one consecutive days. Results; Alcohol ingestion resulted in a significant (p  < 0.05) decrease in water, food intake and marked elevation of lipid peroxidation as assessed by the accumulation of malondialdehyde (MDA) in the reproductive tissues. Precisely, MDA level was elevated in the testis, epididymis, seminal vesicle and prostate gland by 81 %, 63 %, 95 % and 91 %, respectively. Furthermore, levels of total protein, reduced glutathione (GSH), vitamin C and activities of antioxidant enzymes in the reproductive tissues were significantly (p < 0.0001) reduced in ethanol-ingested rats. Interestingly, co-administration of T. conophorum with ethanol led to almost complete inhibition of lipid peroxidation thereby enhancing antioxidant status of the reproductive tissues. Conclusion; Overall, T. conophorum ameliorates oxidative reproductive toxicity induced by ethanol in male rats and its ameliorative effect comparable well with the fertility drug, clomiphene citrate.

Publisher Nil
Publication Type Journal
Publication Title Tetracarpidium Conophorum (Mull.Arg) Hutch & Dalziel Inhibits FeSO4-induced Lipid Peroxidation In Rat's Genitals
Publication Authors Akomolafe SF, Oboh G, Akindahunsi AA, Afolayan AJ
Year Published 2015
Abstract
Background; To search for new sources of safe and inexpensive antioxidant agents which can be used to treat various oxidative stress - related diseases, the phenolic contents of leaf of Tetracarpidium conophorum were characterized and its effect on pro-oxidant induced oxidative stress in rat’s genitals for the first time was investigated. Methods; The aqueous extract of the plant was prepared, the antioxidant activities of the extract were then evaluated using spectrophotometric method. Results; The result revealed that the introduction of aqueous extract of the plant caused significant concentration-dependent decrease (P < 0.05) in the MDA content of the Fe2+ -stressed testes and penis homogenates. The least MDA production occurred at the highest concentration of the extract (0.625 mg/mL). However, characterization of the extract with HPLC revealed that its major constituents were gallic acid, catechin, chlorogenic acid, caffeic acid, coumarin, rutin, quercitrin, quercetin, kaempferol and luteolin. Also, the result revealed that the ABTS* scavenging ability of the extract was 4.60 mmol/100 g while its vitamin C content was 23.49 mg/g which indicated that the plant is very rich in vitamin C. Furthermore, the extract scavenged DPPH, NO, OH* radicals and chelated Fe2+ in a dose-dependent manner. Conclusion; The inhibitory effect of Tetracarpidium conophorum leaves could be attributed to the high levels of quercitrin, quercetin and luteolin and the mechanism through which these compounds possibly do this, could be by their radical scavenging abilities.

Publisher De Gruyter
Publication Type Journal
Publication Title Effect Of Homopterocarpin, An Isoflavonoid From Pterocarpus Erinaceus, On Indices Of Liver Injury And Oxidative Stress In Acetaminophen-provoked Hepatotoxicity.
Publication Authors Afolabi C Akinmoladun · M Tolulope Olaleye · Kayode Komolafe · Abayomi O Adetuyi · Afolabi A Akindahunsi
Year Published 2015
Abstract

Novel hepatoprotectives are needed to address the increasing cases of liver problems worldwide. Pterocarpus erinaceus Poir (Fabaceae) ethanol stem bark extract (PE) and its constituent flavonoid, homopterocarpin (HP), were investigated for their protective property in acetaminophen-induced oxidative stress and liver damage.Adult male albino rats were divided into nine groups. Seven groups were pretreated with PE (50-, 100-, and 150 mg/kg), HP (25-, 50-, and 75 mg/kg) or silymarin (25 mg/kg), respectively, once daily for 5 consecutive days and then administered acetaminophen (2 g/kg) on the 5th day. The control and acetaminophen-intoxicated groups received normal saline throughout the experimental period, with the latter group additionally receiving 2 g/kg acetaminophen on the 5th day. Administrations were performed po.In the acetaminophen-intoxicated group, there were significant increases (p<0.05) in serum activities of alanine aminotransferase (31.72±3.3 vs. 22.1±1.2 U/I), aspartate aminotransferase (185.1±10.1 vs. 103.83±13.3 U/I), bilirubin level and hepatic malondialdehyde (2.32±0.3 vs. 1.42±0.1 units/mg protein), accompanied with significant decreases (p<0.05) in hepatic reduced glutathione level (0.10±0.01 vs. 0.23±0.03 units/mg protein) and glutathione peroxidase activity (2.51±0.2 vs. 3.25±0.2 μmol H2O2 consumed/min/mg protein) compared with the control.PE and HP ameliorated most of the observed biochemical alterations with HP appearing to show more potency. The results suggest that the flavonoid, homopterocarpin contributes to the hepatoprotective and antioxidant potentials of P. erinaceus extract.


Publisher Nil
Publication Type Journal
Publication Title Cytotoxic Flavonoids From The Young Twigs And Leaves Of Caesalpinia Bonduc (Linn) Roxb
Publication Authors Olubanke Olujoke Ogunlana, Wen Jun He, Jun Ting Fan, Guang Zhi Zeng, Chang Jiu Ji, Yu Quing Zheng, Joseph Abayomi Olagunju, Afolabi Akintunde Akindahunsi and Ning Hua Tan
Year Published 2015
Abstract

The extraction, fractionation and recognition of flavonoids from the ethanolic extract of young twigs and leaves of C. bonduc were carried out. In addition, cytotoxic study of the flavonoids on two cancer cell lines, BGC-823 and HeLa was carried our using sulphorhodamine B assay. Seven flavonoids, six of which are being reported for the first time in this plant, were isolated. Their structures were identified by MS and NMR spectroscopic methods. Petroleum ether, ethyl acetate and water fractions exhibited moderate cytotoxic activity against HeLa cells. Five compounds showed cytotoxic activity against HeLa cell in comparison with Paclitaxel, while only one compound showed a good degree of cytotoxic activity against BGC-823 cell in comparison to Paclitaxel. The results obtained showed a structure - activity relationship.


Publisher Elsevier
Publication Type Journal
Publication Title Ethylacetate Extract Of Red Onion (Allium Cepa L.) Tunic Affects Hemodynamic Parameters In Rats.
Publication Authors Olanrewaju Sam Olayeriju, Mary Tolulope Olaleye, Olamide Olajusi Crown, Kayode Komolafe, Aline Augusti Bolignon, Margareth Linde Athayde and Akintunde Afolabi Akindahunsi
Year Published 2015
Abstract
The effects of ethylacetate extract of red onion (Allium cepa) tunic (ACTE) on some hemodynamic and biochemical parameters were evaluated in normotensive albino rats. Blood pressure parameters were determined in anaesthetized rats orally administered ACTE (10-, 20-, or 40 mg/kg) or ramipril (1 mg/kg) once daily for 14 days. Respectively, 10-, 20-, or 40 mg/kg ACTE produced significant (P < 0.01), dose-dependent fall in systolic blood pressure, SBP (21%, 27%, 33%), diastolic blood pressure, DBP (6%, 10%, 16%), pulse pressure, PP (42%, 49%, 56%), mean arterial blood pressure, MAPB (13%, 18%, 23%) and heart rate, HR (4%, 5%, 7%). The highest effective dose (40 mg/kg) compared well with ramipril (1 mg/kg) with regards to SBP (41%), DBP (19%), PP (70%), MABP (29%) and HR (10%). Similar trends (decreases) were recorded for 40 mg/kg ACTE and ramipril, respectively, as regards the activities of serum enzymes: creatine kinase (60% and 65%), ALT (18% and 14%) and ALP (28% and 16%). HPLC fingerprints of the flavonoid-rich ACTE revealed that flavonols: quercetin, quercitrin, isoquercitrin, rutin and kaempferol are the active flavonoids. The results demonstrate the hypotensive effect of A. cepa tunic flavonoids initiating further investigation of their individual or synergistic contribution(s) to the observed effects.

Publisher Nil
Publication Type Journal
Publication Title Antioxidant Potential Of Phenolic-rich Two Varieties Of Nigerian Local Rice And Their Anti-cholinesterase Activities After In Vitro Digestion
Publication Authors Salawu, S.O; O. F. Alao O. F. Faloye A. A. Akindahunsi A. A. Boligon M. L. Athayde,
Year Published 2016
Abstract
Purpose; The purpose of this paper is to focus on the antioxidant properties of two phenolic-rich varieties of Nigerian local rice and their anti-cholinesterase potential after in vitro-simulated gastrointestinal digestion, with the aim of establishing their actual antioxidant and nutraceutical potential after their passage through the gastrointestinal tract upon consumption. Design/methodology/approach; Two local rice varieties, commonly referred to as “Gboko” and “Ofada” rice commonly grown in Benue State, a middle belt region of Nigeria and south western regions of Nigeria, respectively, were locally processed. Each of the processed grains were divided into two portions; one portion was left uncooked, while the second portion was boiled conventionally as eaten, dried and subsequently milled into powder. The milled samples of the raw and boiled rice were treated with acidified methanol to obtain the methanol extracts; another portion of each samples was subjected to in vitro enzyme digestion using standard methods to mimic human digestion; and the third portion was treated using the same scheme of in vitro digestion without the sets of enzyme which was used to serve as a control for enzyme treatment. The quali-quantitative phenolic profiles of the two local varieties were carried out with the aid of high-performance liquid chromatography with diode-array detection (HPLC-DAD) method. The antioxidant potential and anti-cholinesterase action of the methanolic extracts, the simulated in vitro digested model and the enzyme-treated controls of the rice samples were determined using standard methods and data obtained were subjected to ANOVA; the differences of means were separated using Duncan’s multiple range test (DMRT). Findings; The quali-quantitative assessment of phenolic compounds in the two studied local varieties revealed the presence of some phenolic acids and flavonoids, with a decreased level of most of the identified phenolic compounds after boiling. In vitro enzyme-digested rice for both raw and boiled rice samples showed significantly higher total phenolic content, total flavonoid content, ferric-reducing antioxidant power, 2, 2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS+) scavenging and NO− inhibitory activities than the aqueous-methanolic extracts and the enzyme-treated controls. The aqueous-methanolic extracts displayed a higher 1, 1 diphenyl-2-picrylhdrazyl radical scavenging activity and inhibited Fe2+-induced lipid oxidation in rat’s brain and liver homogenate than that displayed by the in vitro enzyme-digested samples. In vitro enzyme-digested and boiled “Gboko” and “Ofada” rice and raw “Ofada” rice have the potential of inhibiting acetylcholine esterase (AChE) activity. While methanolic extracts of raw and boiled “Ofada” and “Gboko” rice exhibited the potential to inhibit butrylcholinesterase activity. The result of this paper indicates that the selected rice varieties possess antioxidant capacities which are better released after the simulated in vitro enzyme digestion; the result also showed the anti-cholinesterase potential of the studied rice grains and, therefore, they can be considered as nutraceutical health supplements. Originality/value; The paper has demonstrated the antioxidant potentials of the phenolic-containing two Nigerian local rice varieties and established their anti-cholinesterase potential after simulated in vitro enzyme digestion.

Publisher Nil
Publication Type Journal
Publication Title Effect Of Ginger And Turmeric Rhizomes On Inflammatory Cytokines Levels And Enzyme Activities Of Cholinergic And Purinergic Systems In Hypertensive Rats.
Publication Authors Akinyemi AJ, Thomé GR, Morsch VM, Bottari NB, Baldissarelli J, de Oliveira LS, Goularte JF, Belló-Klein A, Duarte T, Duarte M, Boligon AA, Athayde ML, Akindahunsi AA, Oboh G, Schetinger MR
Year Published 2016
Abstract
Inflammation exerts a crucial pathogenic role in the development of hypertension. Hence, the aim of the present study was to investigate the effects of ginger (Zingiber officinale) and turmeric (Curcuma longa) on enzyme activities of purinergic and cholinergic systems as well as inflammatory cytokine levels in Nω-nitro-L-arginine methyl ester hydrochloride-induced hypertensive rats. The rats were divided into seven groups (n = 10); groups 1-3 included normotensive control rats, hypertensive (Nω-nitro-L-arginine methyl ester hydrochloride) rats, and hypertensive control rats treated with atenolol (an antihypertensive drug), while groups 4 and 5 included normotensive and hypertensive (Nω-nitro-L-arginine methyl ester hydrochloride) rats treated with 4 % supplementation of turmeric, respectively, and groups 6 and 7 included normotensive and hypertensive rats treated with 4 % supplementation of ginger, respectively. The animals were induced with hypertension by oral administration of Nω-nitro-L-arginine methyl ester hydrochloride, 40 mg/kg body weight. The results revealed a significant increase in ATP and ADP hydrolysis, adenosine deaminase, and acetylcholinesterase activities in lymphocytes from Nω-nitro-L-arginine methyl ester hydrochloride hypertensive rats when compared with the control rats. In addition, an increase in serum butyrylcholinesterase activity and proinflammatory cytokines (interleukin-1 and - 6, interferon-γ, and tumor necrosis factor-α) with a concomitant decrease in anti-inflammatory cytokines (interleukin-10) was observed in Nω-nitro-L-arginine methyl ester hydrochloride hypertensive rats. However, dietary supplementation of both rhizomes was efficient in preventing these alterations in hypertensive rats by decreasing ATP hydrolysis, acetylcholinesterase, and butyrylcholinesterase activities and proinflammatory cytokines in hypertensive rats. Thus, these activities could suggest a possible insight about the protective mechanisms of the rhizomes against hypertension-related inflammation.

Publisher Nil
Publication Type Journal
Publication Title Antidiabetic Effects Of Mangifera Indica Kernel Flour-supplemented Diet In Streptozotocin-induced Type 2 Diabetes In Rats.
Publication Authors Irondi EA, Oboh G, Akindahunsi AA
Year Published 2017
Abstract
Our previous report showed that Mangifera indica kernel flour (MIKF) is a rich source of pharmacologically important flavonoids and phenolic acids; and that its methanolic extract inhibits some key enzymes linked to the pathology and complications of type 2 diabetes (T2D) in vitro. Hence, this study evaluated the antidiabetic effects of 10% and 20% MIKFsupplemented diets in T2D in rats. T2D was induced in rats using a highfat diet (HFD), lowdose streptozotocin (HFD/STZ) model, by feeding the rats with HFD for 2 weeks followed by single dose administration of STZ (40 mg/kg body weight, intraperitoneally). The diabetic rats were later fed the MIKFsupplemented diets, or administered with metformin (25 mg/kg b.w.) for 21 days; the control rats were fed basal diet during this period. Intake of the MIKFsupplemented diets resulted in significant (P < 0.05) improvement in the fasting blood glucose, hepatic glycogen, glycosylated hemoglobin, lipid profile, plasma electrolytes, hepatic and pancreatic malonaldehyde, and the liver function markers of the diabetic rats, compared with the diabetic control rats. The ameliorative effect of 20% MIKFsupplemented was comparable (P > 0.05) with that of metformin administration in the diabetic rats. It is concluded that M. indica kernel flour has antidiabetic effects in T2D rats, and could therefore be a promising nutraceutical therapy for the management of T2D and its associated complications.

Publisher Nil
Publication Type Journal
Publication Title Ethanol-induced Male Infertility: Effects Of Aqueous Leaf Extract Of Tetracarpidium Conophorum. Andrologia.
Publication Authors Akomolafe SF, Oboh G, Akindahunsi AA, Afolayan AJ
Year Published 2017
Abstract

This study investigated the effects of Tetracarpidium conophorum leaf extract on infertility induced by ethanol in male rats. Thirty rats were randomly divided into six groups of five animals each: Group 1 (positive control) received 0.9% saline only; Group 2 (ethanol alone) were given only 30% ethanol orally at 7 ml/kg body weight per day, thrice in a week; groups 3, 4 and 5 were given ethanol and co-treated with 50, 500 and 1,000 mg/kg body weight of leaf extract, respectively, while Group 6 was given ethanol and co-treated with a fertility drug, clomiphene citrate. Ethanol treatment resulted in significant (p < .05) decrease in LDH activity, G-6PDH activity, glycogen content, 3β and 17β HSD activities and testicular and epididymal Zn and Se contents and furthermore decrease in testicular sperm count, viability and marked increment in total sperm abnormalities, rate of sperm analysis parameters and consequently decreased reproductive hormone levels. Interestingly, co-administration of ethanol with either T. conophorum extract or drug almost ameliorated the toxic assault imposed by ethanol on reproductive organs and improved seminal qualities of the rats


Publisher Elsevier
Publication Type Journal
Publication Title Sausage Tree (Kigelia Africana) Flavonoid Extract Is Neuroprotective In AlCl3-induced Experimental Alzheimer’s Disease
Publication Authors John Adeolu Falode Afolabi C. Akinmoladun M. Tolulope Olaleye Afolabi A. Akindahunsi.
Year Published 2017
Abstract

Alzheimer’s disease (AD) is a developmental neurodegenerative disorder for which there is no effective treatment or cure at present. In this study, the neuroprotective properties of a methanol extract of the leaves of Kigelia africana (KAE) and its flavonoid-rich fraction (FKAE) in aluminum chloride (AlCl3)-induced experimental AD was evaluated. Symptoms mimicking AD were induced in male Sprague Dawley rats by administering 17 mg/kg AlCl3, orally, for six consecutive weeks. Pretreatment of animals with 50 and 100 mg/kg KAE or FKAE for two weeks, followed by their co-administration with AlCl3 for a further four weeks ameliorated neurological deficits, cerebral oxidative stress, neurochemical disturbances and histoarchitectural alterations caused by AlCl3 intoxication. The results suggest that KAE and FKAE are promising therapeutic agents for AD.


Publisher Nil
Publication Type Journal
Publication Title Reversal Of Acetaminophen-generated Oxidative Stress And Concomitant Hepatotoxicity By A Phytopharmaceutical Product
Publication Authors Afolabi C. Akinmoladun Kehinde O. Oguntunde Lawrence O. Owolabi Omotayo B. Ilesanmi Joan O. Ogundele M.Tolulope Olaleye Afolabi A. Akindahunsi
Year Published 2017
Abstract

The increasing popularity of herbal medicine and the well-established health benefits of phytochemicals have spurred the multiplicity of nutraceutical and phytopharmaceutical products. In this study, Trévo™, a nutraceutical and phytopharmaceutical product, was evaluated for beneficial effects in acetaminophen-induced hepatic toxicity in Wistar rats. Animals received Trévo™ (1.5 mL/kg, 3.0 mL/kg or 4.5 mL/kg) orally for 14 days. Hepatotoxicity was induced by the oral administration of acetaminophen (2 g/kg), 24 h prior to sacrifice. Biochemical liver function tests, oxidative stress indicators and histoarchitectural changes were evaluated. Acetaminophen administration occasioned significant increase (P < 0.05) in serum bilirubin level and activities of the aminotransferases, alkaline phosphatase, γ-glutamyltransferase and lactate dehydrogenase accompanied by a significant decrease (P < 0.05) in albumin level as well as histopathological alterations in liver sections. Promotion of hepatic oxidative stress by acetaminophen was revealed by significant (P < 0.05) increase in lipid peroxidation, depletion of reduced glutathione, and decrease in superoxide dismutase and catalase activities. Administration of Trévo™ remarkably ameliorated acetaminophen-induced histopathological alterations and changes in serum and tissue biochemical markers. The protective effect of Trévo™ (4.5 mL/kg) was at par with that of Silymarin (25 mg/kg). The present study indicates that Trévo™ has notable salubrious effects.


Publisher Elsevier
Publication Type Journal
Publication Title Possible Involvement Of Membrane Lipids Peroxidation And Oxidation Of Catalytically Essential Thiols Of The Cerebral Transmembrane Sodium Pump As Component Mechanisms Of Iron-mediated Oxidative Stress-linked Dysfunction Of The Pump's Activity
Publication Authors Omotayo TI, Akinyemi GS, Omololu PA, Ajayi BO, Akindahunsi AA, Rocha JB, Kade IJ
Year Published 2015
Abstract

The precise molecular events defining the complex role of oxidative stress in the inactivation of the cerebral sodium pump in radical-induced neurodegenerative diseases is yet to be fully clarified and thus still open. Herein we investigated the modulation of the activity of the cerebral transmembrane electrogenic enzyme in Fe2+-mediated in vitro oxidative stress model. The results show that Fe2+ inhibited the transmembrane enzyme in a concentration dependent manner and this effect was accompanied by a biphasic generation of aldehydic product of lipid peroxidation. While dithiothreitol prevented both Fe2+ inhibitory effect on the pump and lipid peroxidation, vitamin E prevented only lipid peroxidation but not inhibition of the pump. Besides, malondialdehyde (MDA) inhibited the pump by a mechanism not related to oxidation of its critical thiols. Apparently, the low activity of the pump in degenerative diseases mediated by Fe2+ may involve complex multi-component mechanisms which may partly involve an initial oxidation of the critical thiols of the enzyme directly mediated by Fe2+ and during severe progression of such diseases; aldehydic products of lipid peroxidation such as MDA may further exacerbate this inhibitory effect by a mechanism that is likely not related to the oxidation of the catalytically essential thiols of the ouabain-sensitive cerebral electrogenic pump.


Publisher Elsevier
Publication Type Journal
Publication Title Effect Of Dietary Supplementation Of Ginger And Turmeric Rhizomes On Ectonucleotidases, Adenosine Deaminase And Acetylcholinesterase Activities In Synaptosomes From The Cerebral Cortex Of Hypertensive Rats.
Publication Authors Akinyemi, A.J., Thome, G.R., Morsch, V.M., Stefanello, N., Da Costa, P., Cardoso, A., Goularte, J.F., Belló-Klein, A., Akindahunsi, A.A., Oboh, G., Schetinger, M.R.C
Year Published 2016
Abstract

Ginger and turmeric rhizomes are used in folk medicine for the treatment of several cerebrovascular diseases with limited scientific basis for their action. Hence, in this study, we investigate the effects of two Zingiberaceae varieties (ginger and turmeric) on ectonucleotidases (NTPDase and 50-nucleotidase), adenosine deaminase (ADA) and acetylcholinesterase (AChE) activities in synaptosomes of cerebral cortex from l-NAME induced hypertensive rats. The animals were divided into seven groups (n = 10): normotensive control rats; hypertensive rats; hypertensive rats treated with atenolol; normotensive and hypertensive rats treated with 4% supplementation of turmeric and ginger rhizomes, respectively. After 14 days of pre-treatment with both rhizomes the animals were induced with hypertensionby oral administration of L-NAME. The results revealedan increase of ATP and AMP hydrolysis as well as ADA and AChE activities of cerebral cortex synaptosomes in induced rats when compared with the control. The supplementation of both rhizomes prevented these alterations by decreasing ATP and AMP hydrolysis and ADA and AChE activities in cerebral cortex. In conclusion, this study demonstrated that both rhizomes interfere with the purinergic and cholinergic neurotransmission in cerebral cortex of hypertensive rats. Therefore, we can suggest that both rhizomes exert neuroprotective potential under hypertensive state.


Publisher Nil
Publication Type Conference
Publication Title Cassava Processing, Consumption And Human Health. Proceedings CBN First International Scientific Meeting Of The Cassava Biotechnology Network
Publication Authors Adewusi, S.R. A; Akindahunsi, A.A. and Oke, O.L.
Year Published 1993
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Nutritional And Safety Evaluation In The Livestock Industry: A Case Report Of Feed Poisoning. In: (T.A. Afolayan Et Al. Eds) Impact Of Human Activities On The West African Savanna. Proceedings Of The UNESCO Regional Training Workshop Held At Akure, Nigeria
Publication Authors Akindahunsi, A.A. and Adedire, C.O.
Year Published 1995
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Chemical Studies On Two Tropical Wild Yams, Dioscorea Bulbifera And Dioscorea Manganotiana Proceedings Of The 22nd Annual NIFST Conference
Publication Authors Akindahunsi, A.A. and Oboh, G.
Year Published 1998
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Comparative Studies On The Nutritional Quality Of Some Improved Cultivars And Some Market Samples Of Cowpea. ICTP Preprint
Publication Authors Akindahunsi, A.A.
Year Published 1999
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Effect Of Change In Relative Humidity On The Storage Stability Of Micro-fungi Fermented Gari. Proceedings Of The 24th Annual NIST Conference
Publication Authors Akindahunsi, A.A. and Oboh, G.
Year Published 2000
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Comparative Analysis Of Some Vertebrate 2xRBD+Gly-rich HnRNP Proteins. Proceedings Of The XIIth International Congress On “Genes, Gene Families And Isozymes”
Publication Authors Akindahunsi, A. A., Bandiera, A. and Manzini, G
Year Published 2003
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Safety Evaluation Of Aspergillus Flavus Fermented Cassava Flour (Manihot Esculenta, Crantz). In Proceedings Of 16th Annual Conference Of Biotechnology Society Of Nigeria
Publication Authors Oboh G. and Akindahunsi, A.A
Year Published 2003
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Hypotensive Activities Of Some Nigerian Medicinal Plants Used In The Treatment Of Hypertension. In Proceedings Of 16th Annual Conference Of Biotechnology Society Of Nigeria
Publication Authors Olaleye, M.T., Akindahunsi, A.A. and Ekperigin, M.M
Year Published 2003
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Chemical Hazards Associated With Traditional Methods Of Cassava Processing In Ogun, Ondo And Oyo States And Improved Management Practices. In: Proceedings Of A Workshop On Promotion Of Improved Management Technologies Aimed At Reducing Occupational And Environmental Hazards Associated With Traditional Cassava Processing In Ogun, Ondo And Oyo States, Nigeria
Publication Authors Akindahunsi, A.A and Olaleye, M.T.
Year Published 2004
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Effects Of Partially Purified Extract Of Hibscus Sabdariffa On Blood Pressure Of Normotensive Albino Rats And Some Biochemical Indices. In Proceeding Of International Conference Held In The Federal University Of Technology, Akure , Nigeria
Publication Authors Olaleye, M.T., Akindahunsi, A.A
Year Published 2006
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title HPLC/DAD/MS Qualitative And Quantitative Evaluation Of Phenolic Compounds In Occimum Graticimum And Manihot Utilissima Leave Presented At Akure- Humboldt Kellog/3rd School Of Agriculture And Agricultural Technology, FUTA
Publication Authors Salawu, S.O, Akindahunsi A.A, Giaccherini C, Innocenti M, Vincieri, F. F, and Mulinacci N
Year Published 2007
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title HPLC/DAD/MS Analysis Of Flavonoids And Cynamoyl Derivatives In Vernonia Amygdalina. ICTP Preprint
Publication Authors Salawu, S.O, Giaccherini C, Innocenti M, Vincieri, F. F, Akindahunsi, A. A and Mulinacci N
Year Published 2007
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title HPLC/DAD/MS Analysis Of Flavonoids And Cynamoyl Derivatives In Vernonia Amygdalina Leaves Presented At Science Association Of Nigeria (SAN).
Publication Authors Salawu, S.O, Akindahunsi A.A, Giaccherini C, Innocenti M, Vincieri, F. F, and Mulinacci N
Year Published 2007
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Polyphenolic Screening And Protective Properties Of Some Vegetables Against CCl4 Liver Damage. ICTP Preprint
Publication Authors Salawu, S.O and Akindahunsi, A.A
Year Published 2007
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Antimicrobial Effects Of Different Extracts From Nigerian Vegetables Consisting Of Flavonoids And Cynamoyl Derivatives. Polyphenols. Communication At Xxivth International Conference On Polyphenols
Publication Authors Salawu, S.O, Giaccherini C, Innocenti M, Vincieri, F. F, Akindahunsi, A. A and Mulinacci N
Year Published 2008
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Cellular Antioxidant Activities And Cytotoxic Properties Of Phenolic Containing Extracts Of Four Nigerian Green Leafy Vegetables. Polyphenols Communication At Xxvth International Conference On Polyphenols
Publication Authors Salawu, S.O, Decorti G, Cvorovic J, Tramer, F., Passamonti, S, Akindahunsi, A. A and Mulinacci N
Year Published 2010
Abstract

Nil


Publisher Nil
Publication Type Technical Papers
Publication Title Mitochondrial Nutrients And Parkinson’s Disease Chemotherapy. Second Annual Faculty Lecture Of The School Of Sciences, Federal University Of Technology, Akure (FUTA), Nigeria
Publication Authors Akindahunsi, A. A
Year Published 2006
Abstract

Nil


Publisher Nil
Publication Type Technical Papers
Publication Title Prospects Of Biochemistry In The 21st Century. Paper Presented At The National Association Of Biochemistry Students (OAU Chapter) Week Held At Obafemi Awolowo University, Ile-Ife
Publication Authors Akindahunsi A. A
Year Published 2007
Abstract

Nil


Publisher Nil
Publication Type Technical Papers
Publication Title Food Attitude And The Incidence Of Oxidative Stress. Invited Paper Presented At The 38th Annual Conference And Scientific Meeting, Nutrition Society Of Nigeria
Publication Authors Akindahunsi A. A
Year Published 2007
Abstract

Nil


Publisher Nil
Publication Type Technical Papers
Publication Title Research And Publications: The Global Perspective. Invited Paper Presented At The University Library Seminar Series, The Federal University Of Technology, Akure
Publication Authors Akindahunsi A. A
Year Published 2007
Abstract

Nil


Publisher Nil
Publication Type Technical Papers
Publication Title Chemistry Of Oxidative Stress. Invited Paper Presented At The Lecture Series Of The Chemistry Department, University Of Ibadan
Publication Authors Akindahunsi, A. A
Year Published 2008
Abstract

Nil


Publisher Nil
Publication Type Technical Papers
Publication Title Ethics In Research, A Paper Presented At A 3-Day Training Workshop On Proposal Development And Methodology For Need-Driven Research: Contemporary Issues On Grantsmanship (13-15 January, 2009) Organized By Centre For Research And Development (CERAD), The Federal University Of Technology, Akure
Publication Authors Akindahunsi A. A
Year Published 2009
Abstract

Nil


Publisher Nil
Publication Type Conference
Publication Title Ethics In Research, A Paper Presented At A 3-Day Training Workshop On Proposal Development And Methodology For Need-Driven Research: Contemporary Issues On Grantsmanship (21-23 March, 2011) Organized By Centre For Research And Development (CERAD), The Federal University Of Technology, Akure
Publication Authors Akindahunsi A. A
Year Published 2011
Abstract

Nil


Publisher Nil
Publication Type Technical Papers
Publication Title Research Methods In Pure And Applied Sciences, A Paper Presented At A 3-Day Training Workshop On Research Methodology And Statistical Analysis (27-29 November, 2012) Organized By Centre For Research And Development (CERAD), The Federal University Of Technology, Akure
Publication Authors Akindahunsi A. A
Year Published 2012
Abstract

Nil


Publisher Nil
Publication Type Technical Papers
Publication Title From Problem Identification To Formulation Of Research Questions And Objectives, A Paper Presented At A 3-Day Training Workshop On Grantsmanship Proposal Writing (10-12 May, 2016), Organized By Centre For Research And Development (CERAD), The Federal University Of Technology, Akure
Publication Authors Akindahunsi A. A
Year Published 2016
Abstract

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Publisher Nil
Publication Type Technical Papers
Publication Title Molecular Biology In Agriculture And Food Security. A Lead Paper Presented At The 2016 Annual Conference Of The Nigerian Society Of Biochemistry And Molecular Biology
Publication Authors Akindahunsi A. A
Year Published 2016
Abstract

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